Jensen I M, Hokland P
Arhus University Hospital, Department of Haematology and Medicine, Arhus Amtssygehus, Denmark.
Br J Haematol. 1994 Jul;87(3):477-82. doi: 10.1111/j.1365-2141.1994.tb08301.x.
Double staining of bone marrow cells for CD13 and CD33 leucocyte differentiatian antigens and for DNA content has allowed us to evaluate the proliferative capacity of myelopoiesis in patients with myelodysplastic syndromes (MDS) using flow cytometry. By analysing 39 patients (15 RA/RAS, 14 RAEB and 10 RAEB-t) and eight normal controls, we found significant differences in both the percentage of cells positive for these immature myeloid antigens between the FAB groups as well as in the fractions of CD13 and CD33 positive cells in S or S-G2M phase of the cell cycle. Moreover, a clear decrease in the immature myeloid cell proliferative activity upon progression within the FAB groups was evident. Finally, we found a significant negative association between the percentage of myeloblasts in the bone marrow and the proliferative activity of the immature myeloid cells, indicating that the block in differentiation in MDS patients might be coupled to a simultaneous block in proliferation, especially in advanced stages. These data suggest that the use of double parameter assays in the longitudinal follow-up of MDS patients might yield new information about the biology of MDS.
通过对骨髓细胞进行CD13和CD33白细胞分化抗原以及DNA含量的双重染色，我们得以运用流式细胞术评估骨髓增生异常综合征（MDS）患者骨髓生成的增殖能力。通过分析39例患者（15例RA/RAS、14例RAEB和10例RAEB-t）以及8名正常对照，我们发现，在FAB各亚组之间，这些未成熟髓系抗原阳性细胞的百分比，以及细胞周期S期或S-G2M期CD13和CD33阳性细胞的比例均存在显著差异。此外，在FAB各亚组中，随着病情进展，未成熟髓系细胞的增殖活性明显降低。最后，我们发现骨髓中原始粒细胞的百分比与未成熟髓系细胞的增殖活性之间存在显著的负相关，这表明MDS患者的分化阻滞可能与同时存在的增殖阻滞相关，尤其是在疾病晚期。这些数据表明，在MDS患者的长期随访中使用双参数检测可能会产生有关MDS生物学特性的新信息。
