Seigneurin D, Louis J, Villoud M C
Quantitative Cytology Group, Faculty of Medicine, La Tronche, France.
Anal Cell Pathol. 1994 Aug;7(2):115-25.
Cytological evaluation of breast diseases can lead to false diagnosis if benign lesions show epithelial atypia or if carcinomas are orthoplasic and well differentiated. In order to determine the potential usefulness of image cytometry for discrimination between benign and malignant breast lesions, we studied 120 Feulgen-stained imprints, half from benign lesions with epithelial atypia and half from well-differentiated carcinomas and determined values of parameters expressing DNA content, morphometry and chromatin texture. Twenty-five out of the 60 carcinoma imprints were found to be DNA-aneuploid and could thus be easily distinguished from benign imprints, as, in our and other authors' experiences, aneuploidy very rarely occurs in benign breast lesions. When the remaining 35 carcinoma imprints were compared with 38 benign lesion imprints, randomly selected from the 60 cases studied, only four cases were misclassified after a linear discriminant analysis; the most discriminant parameters were those expressing the heterogeneity of DNA content and the chromatin texture. Thus, in cases where cytological diagnosis is difficult, image analysis of Feulgen-stained imprints may be a very useful tool for correctly discriminating benign from malignant cases.
如果良性病变表现出上皮异型性，或者癌为规整性且高分化，那么乳腺疾病的细胞学评估可能会导致误诊。为了确定图像细胞术在鉴别乳腺良恶性病变方面的潜在用途，我们研究了120张福尔根染色的印片，其中一半来自伴有上皮异型性的良性病变，另一半来自高分化癌，并确定了表达DNA含量、形态测量和染色质纹理的参数值。在60张癌印片中，有25张被发现为DNA非整倍体，因此很容易与良性印片区分开来，因为根据我们和其他作者的经验，非整倍体在良性乳腺病变中很少发生。当将其余35张癌印片与从所研究的60例病例中随机选取的38张良性病变印片进行比较时，经过线性判别分析后，只有4例被错误分类；最具判别力的参数是那些表达DNA含量异质性和染色质纹理的参数。因此，在细胞学诊断困难的情况下，福尔根染色印片的图像分析可能是正确鉴别良性和恶性病例的非常有用的工具。
