Schenck U, Burger G, Jütting U, Schenck U B, Wagner A, Breul J
Institut für Allgemeine Pathologie und Pathologische Anatomie, Technischen Universität München, Germany.
Anal Cell Pathol. 1994 Aug;7(2):139-52.
Until the present it was not possible to predict hormone sensitivity of prostatic carcinoma. Based on studies correlating image cytometric results of hormone receptor negative and hormone receptor positive breast carcinomas, the present study aims at separating responders and non-responders to hormone therapy in metastatic prostatic carcinoma. From May-Grünwald-Giemsa stained slides of fine needle aspirates of 23 patients with metastasizing prostatic carcinoma about 100 nuclei per slide were taken by TV camera for image-cytometric processing. One thousand and twenty-two nuclei came from 10 patients who showed tumour regression for at least 36 months and who all survived for more than 5 years. One thousand three hundred and thirty-two nuclei were from prostatic aspirates of patients who showed a continuous tumour progression despite receiving hormone therapy. All patients of the latter group died within 5 years. A correct classification of the patient groups of responders and non-responders was possible in 19-21 of 23 cases by means of high resolution image analysis including nuclear structural features. It was found that even simple planimetric features, like the nuclear perimeter, or densitometric features, such as the total nuclear extinction, differed markedly between the two groups. The data show that nuclei from hormone sensitive prostatic carcinoma are distinct from those of non-sensitive ones in the present series. The interpretation of results must take into account that the very strict criteria for hormone sensitivity leads to a highly selected patient group. The application of the method to an unselected patient group can be presumed to yield a higher rate of false classifications.
直到目前，前列腺癌的激素敏感性仍无法预测。基于对激素受体阴性和激素受体阳性乳腺癌的图像细胞计量结果进行的相关性研究，本研究旨在区分转移性前列腺癌患者对激素治疗的反应者和无反应者。从23例转移性前列腺癌患者的细针穿刺涂片经May-Grünwald-Giemsa染色的玻片上，用电视摄像机每张玻片采集约100个细胞核进行图像细胞计量处理。1022个细胞核来自10例肿瘤消退至少36个月且均存活超过5年的患者。1332个细胞核来自尽管接受了激素治疗但仍出现肿瘤持续进展的患者的前列腺穿刺物。后一组的所有患者均在5年内死亡。通过包括核结构特征在内的高分辨率图像分析，在23例中的19 - 21例中能够正确区分反应者和无反应者这两组患者。研究发现，即使是简单的平面测量特征，如核周长，或密度测量特征，如总核消光，在两组之间也有显著差异。数据表明，在本系列中，激素敏感型前列腺癌的细胞核与非敏感型的细胞核不同。对结果的解释必须考虑到，对激素敏感性的非常严格的标准导致了一个高度选择的患者群体。可以推测，将该方法应用于未选择的患者群体将会产生更高的错误分类率。
