Tomii S, Kinoshita S
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.
Scanning. 1994 Sep-Oct;16(5):305-6.
We applied the tandem scanning confocal microscope (TSCM) to 30 healthy human corneas of 3 normal volunteers and 27 patients with cataract and retinal detachment to observe normal corneal epithelial cells in vivo. All corneas were normal under slit lamp microscopic examination. The superficial and basal epithelial cells close to the basal lamina in the central cornea were recorded on videotape and analyzed by a computer-assisted digitizer. The mean cell areas of superficial cells exposed at the surface and basal cells at the horizontal section were 624 +/- 109 microns2 and 66 +/- 5 microns2, respectively. The ratio of superficial to basal mean cell area was 11.0 +/- 4.5. TSCM was thus useful in evaluating the relationship between superficial and basal cells in human corneal epithelium in vivo.
我们将串联扫描共聚焦显微镜（TSCM）应用于3名正常志愿者的30只健康人角膜以及27例白内障和视网膜脱离患者的角膜，以在体内观察正常角膜上皮细胞。所有角膜在裂隙灯显微镜检查下均正常。对中央角膜靠近基底膜的表层和基底上皮细胞进行录像，并通过计算机辅助数字化仪进行分析。表面暴露的表层细胞和水平截面的基底细胞的平均细胞面积分别为624±109平方微米和66±5平方微米。表层与基底平均细胞面积之比为11.0±4.5。因此，串联扫描共聚焦显微镜在评估人角膜上皮表层和基底细胞之间的关系方面很有用。
