氧化还原酶和水解酶作为大鼠胰岛超离心的标记酶。

Oxidoreductases and hydrolases as marker enzymes for ultracentrifugation of islets of Langerhans of rats.

作者信息

Jahr H, Zühlke H

出版信息

Acta Biol Med Ger. 1976;35(11):1477-86.

Abstract

Using quantitative fluorometric micro methods the presence of glutamate dehydrogenase, acid galactosidase, and acid glucuronidase was detected in pancreatic islets of the rat. Some properties of these enzymes and of malate dehydrogenase, 6-phosphogluconate dehydrogenase, and acid phosphatase were investigated. It has been shown that subcellular fractions of homogenates of islets of Langerhans can be characterized by using glutamate dehydrogenase, 6-phosphogluconate dehydrogenase, and acid hydrolases as marker enzymes for mitochondria, cytosol, and lysosomes, respectively. The degree of contamination from acinar tissue in the islet preparations was calculated from the amylase activity of the homogenates.

摘要

采用定量荧光显微法在大鼠胰岛中检测到谷氨酸脱氢酶、酸性半乳糖苷酶和酸性葡萄糖醛酸酶的存在。对这些酶以及苹果酸脱氢酶、6-磷酸葡萄糖酸脱氢酶和酸性磷酸酶的一些特性进行了研究。结果表明，通过分别使用谷氨酸脱氢酶、6-磷酸葡萄糖酸脱氢酶和酸性水解酶作为线粒体、胞质溶胶和溶酶体的标记酶，可以对胰岛匀浆的亚细胞组分进行表征。根据匀浆的淀粉酶活性计算胰岛制剂中腺泡组织的污染程度。