促甲状腺激素释放激素原激素的一种神秘肽抑制促甲状腺激素释放激素诱导的生长激素释放。
A cryptic peptide of TRH prohormone inhibits TRH-induced GH release.
作者信息
Roussel J P, Teresi S, Vaudry H, Astier H
机构信息
Laboratoire de Neurobiologie Endocrinologique, URA 1197 CNRS, Université de Montpellier II, France.
出版信息
C R Acad Sci III. 1994 Mar;317(3):270-6.
The effects of two cryptic peptides from pro-TRH: Ps4 (160-169) and Ps5 (178-199) were investigated on basal and secretagogue (GRH and TRH)-induced releases of GH from perifused fragments of rat adenohypophysis. Validation of the perifusion system was done by measuring: (1) the dose-dependent effect of GRH and TRH on GH release; and (2) the stimulation of that release by forskolin (to mimic the adenylate cyclase pathway) or by phorbol ester (to mimic the protein kinase C pathway). We show that: (1) Ps4 and Ps5 (1 microM) do not modify basal GH release; (2) Ps4 (1 microM) changes neither GRH (10 nM)- nor TRH (100 nM)-induced release of GH; (3) Ps5 (100 nM and 1 microM) significantly decreases the release of GH induced by equimolar concentrations of TRH but not that induced by GRH.
研究了来自促甲状腺激素释放激素原的两种隐蔽肽
Ps4（160 - 169）和Ps5（178 - 199）对大鼠腺垂体灌流片段基础状态下以及促分泌素（生长激素释放激素和促甲状腺激素释放激素）诱导的生长激素释放的影响。通过测量以下指标对灌流系统进行验证：（1）生长激素释放激素和促甲状腺激素释放激素对生长激素释放的剂量依赖性效应；（2）福斯可林（模拟腺苷酸环化酶途径）或佛波酯（模拟蛋白激酶C途径）对该释放的刺激作用。我们发现：（1）Ps4和Ps5（1微摩尔）不改变基础状态下的生长激素释放；（2）Ps4（1微摩尔）既不改变生长激素释放激素（10纳摩尔）也不改变促甲状腺激素释放激素（100纳摩尔）诱导的生长激素释放；（3）Ps5（100纳摩尔和1微摩尔）显著降低等摩尔浓度促甲状腺激素释放激素诱导的生长激素释放，但不降低生长激素释放激素诱导的生长激素释放。
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