Duclos A J, Pomerantz D K, Baines M G
Department of Microbiology and Immunology, McGill University, Montréal, Québec, Canada.
Cell Immunol. 1994 Dec;159(2):184-93. doi: 10.1006/cimm.1994.1306.
The relationship between the early cellular response to embryo implantation and subsequent embryo survival was explored. Immunohistochemistry using the anti-CD11b antibody (Mac-1) was used to localize and quantify maternal inflammatory cells present at the fetoplacental interface. CD 11b is expressed mostly on macrophages, but is also present on natural killer (NK) cells, neutrophils, and B cells. The occurrence of CD11b-positive cells at the fetoplacental interface was quantified in CBA/J females mated by DBA/2 males (20-30% embryo loss) and CBA/J females mated by BALB/c males (5-10% embryo loss) in order to investigate the relationship between infiltration by these types of cells and subsequent embryo loss. CD11b-positive cells were found to infiltrate decidua of each embryo starting at Day 6 of gestation. Their numbers sharply increased on Days 7 and 8, to a plateau on Days 8 to 10, well before any damage to the embryo is macroscopically visible on Days 10 to 12 of gestation. The resorption-prone mating of CBA/J female by DBA/2 male showed a significantly elevated number of CD11b-positive cells in 26% of the embryos on the eighth day of gestation compared to CBA/J female by BALB/c male matings which were taken as the reference mating. Moreover, experimental conditions modulating fetal survival in CBA/J mothers such as poly (I:C) treatment of DBA/2-mated females (lower survival) or mating with BALB/c males (higher survival than with the mating with DBA/2 males), were found to be associated with high or low numbers numbers of CD11b-positive cells at the fetoplacental interface. Furthermore, injection of anti-CD 11b into pregnant mice at Day 6 of gestation significantly reduced the subsequent incidence of resorption in the resorption prone CBA/J x DBA/2 mating. These results suggest that CD11b-positive cells are associated with the etiology of spontaneous abortion in this system.
研究了胚胎着床早期细胞反应与后续胚胎存活之间的关系。使用抗CD11b抗体（Mac-1）的免疫组织化学方法来定位和定量存在于胎盘界面的母体炎症细胞。CD11b主要在巨噬细胞上表达，但也存在于自然杀伤（NK）细胞、中性粒细胞和B细胞上。为了研究这些类型的细胞浸润与后续胚胎丢失之间的关系，对与DBA/2雄性交配的CBA/J雌性（胚胎丢失率为20-30%）和与BALB/c雄性交配的CBA/J雌性（胚胎丢失率为5-10%）胎盘界面处CD11b阳性细胞的出现情况进行了定量。发现CD11b阳性细胞从妊娠第6天开始浸润每个胚胎的蜕膜。它们的数量在第7天和第8天急剧增加，在第8至10天达到平台期，远早于在妊娠第10至12天胚胎出现任何肉眼可见的损伤之前。与作为参考交配的CBA/J雌性与BALB/c雄性交配相比，CBA/J雌性与DBA/2雄性的易吸收交配在妊娠第8天26%的胚胎中显示出CD11b阳性细胞数量显著增加。此外，发现调节CBA/J母亲胎儿存活的实验条件，如对与DBA/2交配的雌性进行聚（I:C）处理（存活率较低）或与BALB/c雄性交配（存活率高于与DBA/2雄性交配），与胎盘界面处CD11b阳性细胞数量的高或低有关。此外，在妊娠第6天向怀孕小鼠注射抗CD11b可显著降低易吸收的CBA/J×DBA/2交配中随后的吸收发生率。这些结果表明，CD11b阳性细胞与该系统中自然流产的病因有关。
