Katano M, Morisaki T, Matsuo T, Nakamura M, Kubota E, Hisatsugu T
Department of Surgery, Saga Medical School, Japan.
Cell Immunol. 1994 Dec;159(2):262-70. doi: 10.1006/cimm.1994.1312.
A human B-cell line (Hairy-BM) constitutively secreting interleukin-2 (IL-2) was established from tumor tissue resected surgically from a patient with breast cancer. Hairy-BM was found to be 100% CD20+, 98% surface immunoglobulin (sIg) G+, 98% sIg kappa chain+, 100% HLA-DR+, 94% IL-2 receptor (IL-2R alpha), 98% IL-2R beta, and devoid of T-cell, monocyte, and natural killer cell surface antigens. The B-cell origin of Hairy-BM was also confirmed by clonally rearranged Ig heavy- and Ig light-chain genes. The growth of Hairy-BM expressing IL-2R was promoted by recombinant IL-2 (rIL-2) and anti-CD25 antibody significantly blocked the growth enhancement. IL-2 secretion from Hairy-BM was confirmed by radioimmunoassay. By using a sensitive polymerase chain reaction technique, we demonstrated that Hairy-BM expressed IL-2 mRNA, IL-2R alpha mRNA, and IL-2R beta mRNA. These findings indicate that certain B-cells not only produce, but also respond to IL-2 in an autocrine fashion with increased proliferation.
从一名乳腺癌患者手术切除的肿瘤组织中建立了一种持续分泌白细胞介素-2（IL-2）的人B细胞系（Hairy-BM）。发现Hairy-BM 100% CD20阳性、98%表面免疫球蛋白（sIg）G阳性、98% sIg κ链阳性、100% HLA-DR阳性、94%白细胞介素-2受体（IL-2Rα）、98% IL-2Rβ，且缺乏T细胞、单核细胞和自然杀伤细胞表面抗原。Hairy-BM的B细胞起源也通过克隆重排的Ig重链和Ig轻链基因得到证实。重组IL-2（rIL-2）促进了表达IL-2R的Hairy-BM的生长，抗CD25抗体显著阻断了生长增强。通过放射免疫测定证实了Hairy-BM分泌IL-2。通过使用灵敏的聚合酶链反应技术，我们证明Hairy-BM表达IL-2 mRNA、IL-2Rα mRNA和IL-2Rβ mRNA。这些发现表明某些B细胞不仅产生IL-2，而且以自分泌方式对IL-2作出反应，增殖增加。
