裸鼠传代鼻咽癌组织中EB病毒BamHI F DNA片段的分析

Analysis of Epstein-Barr virus BamHI F DNA fragment in nude mouse-passaged nasopharyngeal carcinoma tissues.

作者信息

Liu M Y, Lin S F, Hsu T Y, Chen J Y, Yang C S

机构信息

Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Republic of China.

出版信息

Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1993 May;26(2):68-77.

PMID:7995078

Abstract

Two nude mouse-passaged nasopharyngeal carcinoma (NPC) tissues, NPC-295 and NPC-306, were different in ability to transform cord blood lymphocytes and contained Epstein-Barr virus (EBV) genomes with difference in BamHI F DNA fragment. Four clones containing DNA sequences of the BamHI F fragment (54,853-62,249) were obtained from genomic libraries of NPC-295 and NPC-306 and their partial restriction enzyme maps and sequences were determined. The restriction enzyme maps of EBV DNA at the BamHI F fragment in NPC-295 and NPC-306 appeared to be similar to that of EBV B95-8 strain. However, 8 nucleotide differences were revealed between NPC-295 and the B95-8 strain when 566 nucleotides (55,405-55,970) were compared, and 15 out of 677 nucleotides analyzed (55,410-56,086) were different between NPC-306 and B95-8.

摘要

两株经裸鼠传代的鼻咽癌（NPC）组织，NPC - 295和NPC - 306，在转化脐血淋巴细胞的能力上有所不同，并且含有EB病毒（EBV）基因组，其BamHI F DNA片段存在差异。从NPC - 295和NPC - 306的基因组文库中获得了四个包含BamHI F片段（54,853 - 62,249）DNA序列的克隆，并确定了它们的部分限制性酶切图谱和序列。NPC - 295和NPC - 306中EBV DNA在BamHI F片段处的限制性酶切图谱似乎与EBV B95 - 8株的相似。然而，当比较566个核苷酸（55,405 - 55,970）时，NPC - 295与B95 - 8株之间显示出8个核苷酸差异，在分析的677个核苷酸（55,410 - 56,086）中，NPC - 306与B95 - 8之间有15个不同。