Janowski T
Department of Obstetrics, Faculty of Veterinary Medicine, Academy of Agriculture and Technology, Olsztyn, Poland.
Exp Clin Endocrinol. 1994;102(3):193-4. doi: 10.1055/s-0029-1211282.
The aim of this study was to determine if bovine placenta is capable of producing the gestagen metabolites in vivo. The concentrations of 5 beta-pregnane-3 alpha-20 alpha-diol, 17 alpha,20 alpha-dihydroxyprogesterone and progesterone were measured by HPLC-RIA in samples obtained after surgical preparation from peripheral, uterine, fetal and ovarian blood of seven cows near term. Measurement of arterio-venous differences in maternal uterine and umbilical circulation confirmed the placental origin of 5 beta-pregnance-3 alpha,20 alpha-diol and 17 alpha,20 alpha-dihydroxyprogesterone, but not progesterone itself. 5 beta-pregnane-3 alpha,20 alpha-diol was preferentially secreted into the fetal compartment. The present experiment, according to earlier in-vitro studies, confirms that the preterm bovine placenta is qualified for in-vivo utilization and secretion of gestagen metabolites.
本研究的目的是确定牛胎盘是否能够在体内产生孕激素代谢产物。通过高效液相色谱 - 放射免疫分析法（HPLC - RIA）测定了7头接近足月的奶牛在手术准备后从外周血、子宫血、胎儿血和卵巢血中获取的样本中5β - 孕烷 - 3α - 20α - 二醇、17α,20α - 二羟基孕酮和孕酮的浓度。对母体子宫和脐循环中动静脉差异的测量证实了5β - 孕烷 - 3α,20α - 二醇和17α,20α - 二羟基孕酮的胎盘来源，但不包括孕酮本身。5β - 孕烷 - 3α,20α - 二醇优先分泌到胎儿体内。根据早期的体外研究，本实验证实早产牛胎盘有资格在体内利用和分泌孕激素代谢产物。
