Dentener M A, Smit F T, Francot G J, Buurman W A
Department of Surgery, University of Limburg, Maastricht, Netherlands.
J Infect Dis. 1994 Dec;170(6):1483-9. doi: 10.1093/infdis/170.6.1483.
In this study the production and characterization of two monoclonal antibodies (MAbs), 4E3 and 5D7, against the bactericidal/permeability-increasing protein (BPI) were described. Using ELISAs, both 4E3 and 5D7 were shown to detect recombinant (r) BPI. Furthermore, natural BPI present in polymorphonuclear leukocytes (PMNL) was detected by both 4E3 and 5D7. The use of both MAbs in flow cytometry revealed that PMNL expressed low levels of cell-surface BPI. Lipopolysaccharide (LPS) was shown to block the interaction between anti-BPI MAb and rBPI. In addition, the MAbs blocked biologic activity of rBPI. The inhibition by BPI of LPS activation of the limulus amebocyte lysate assay and of LPS-induced tumor necrosis factor-alpha release by monocytes was prevented by 4E3 and 5D7. Both MAbs are specifically directed against BPI and can inhibit BPI bioactivity.
本研究描述了两种抗杀菌/通透性增加蛋白（BPI）的单克隆抗体（MAb）4E3和5D7的制备及特性。通过酶联免疫吸附测定（ELISA）显示，4E3和5D7均可检测重组（r）BPI。此外，4E3和5D7均能检测到多形核白细胞（PMNL）中存在的天然BPI。在流式细胞术中使用这两种单克隆抗体表明，PMNL表达低水平的细胞表面BPI。脂多糖（LPS）可阻断抗BPI单克隆抗体与rBPI之间的相互作用。此外，这些单克隆抗体还可阻断rBPI的生物学活性。4E3和5D7可阻止BPI对鲎试剂法中LPS激活的抑制作用以及对单核细胞LPS诱导的肿瘤坏死因子-α释放的抑制作用。这两种单克隆抗体均特异性针对BPI，并可抑制BPI的生物活性。
