不同来源的大肠杆菌O44:H18分离株具有聚集性。

Isolates of Escherichia coli O44:H18 of diverse origin are enteroaggregative.

作者信息

Smith H R, Scotland S M, Willshaw G A, Rowe B, Cravioto A, Eslava C

机构信息

Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, United Kingdom.

出版信息

J Infect Dis. 1994 Dec;170(6):1610-3. doi: 10.1093/infdis/170.6.1610.

DOI:10.1093/infdis/170.6.1610

PMID:7996006

Abstract

One hundred thirteen strains of Escherichia coli O44:H18 isolated in several countries over 25 years were examined for adhesion to tissue culture cells and for hybridization with DNA probes. Fifty-nine strains were from sporadic cases of infection; 54 were from 12 outbreaks. Of the 113 strains, 85 showed aggregative adhesion to HEp-2 cells; 36 were from sporadic cases and 49 were from 9 outbreaks. All adhesive strains hybridized with the probe for enteroaggregative E. coli (EAggEC) and 1 nonadhesive strain was also positive. However, 80 of the 86 EAggEC probe-positive strains also hybridized with the probe for diffusely adherent E. coli (DAEC) derived from the daaC gene of strain F1845. The EAggEC and DAEC probes hybridized to different fragments of a large plasmid in the O44:H18 strains.

摘要

对25年间在多个国家分离出的113株大肠杆菌O44:H18进行了检测，以观察其对组织培养细胞的黏附情况以及与DNA探针的杂交情况。59株来自散发性感染病例；54株来自12起疫情。在这113株菌株中，85株对HEp-2细胞表现出聚集性黏附；36株来自散发性病例，49株来自9起疫情。所有黏附性菌株均与肠聚集性大肠杆菌（EAggEC）探针杂交，1株非黏附性菌株也呈阳性。然而，86株EAggEC探针阳性菌株中的80株也与源自F1845菌株daaC基因的弥漫性黏附性大肠杆菌（DAEC）探针杂交。EAggEC和DAEC探针与O44:H18菌株中大质粒的不同片段杂交。