Kasatori N, Urayama T, Mori T, Ishikawa F
Department of Laboratory Medicine, Toho University School of Medicine, Tokyo.
Rinsho Byori. 1994 Oct;42(10):1050-4.
Assay of 51Cr release from target cells has been commonly used in various methods of examining the cytotoxic properties of lymphocytes. In this paper a non-isotopic assay of cytotoxicity based on the leak of endogenous alkaline phosphatase (AIP) in target cells, is described. Enzyme activities were assayed by the luminescence on hydrolysis of the lumigen-PPD substrate. P3-X63-Ag8-U1 (P3U-1) cells were demonstrated to contain AIP and proved sensitive to the IL-2-induced killer lymphocytes, while no AIP activity was detected in human effector lymphocytes. Comparative studies of the test with 51Cr- and AIP-release in P3U-1 target cells were carried out, and the results obtained suggested that the AIP release test is useful as a new, simple lymphocyte cytotoxicity test.
检测靶细胞中51Cr的释放已普遍用于各种检测淋巴细胞细胞毒性特性的方法中。本文描述了一种基于靶细胞内源性碱性磷酸酶（AIP）泄漏的非同位素细胞毒性检测方法。通过鲁米诺-PPD底物水解时的发光来检测酶活性。已证明P3-X63-Ag8-U1（P3U-1）细胞含有AIP，并被证明对IL-2诱导的杀伤淋巴细胞敏感，而在人效应淋巴细胞中未检测到AIP活性。对P3U-1靶细胞中51Cr释放和AIP释放检测进行了比较研究，所得结果表明AIP释放检测作为一种新的、简单的淋巴细胞细胞毒性检测方法是有用的。
