Maxson R T, Dunlap J P, Tryka F, Jackson R J, Smith S D
Department of Surgery, University of Arkansas for Medical Sciences, Little Rock.
J Surg Res. 1994 Dec;57(6):682-6. doi: 10.1006/jsre.1994.1201.
The mucus gel layer is thought to be a vital component of the intestinal mucosal barrier. The purpose of this study is to determine if decreasing mucus production following ischemia and reoxygenation of the intestinal mucosa would alter bacterial translocation in an in vitro rat mucosal model. Clonidine was used to decrease mucus production associated with an ischemia/reoxygenation insult. Bacterial translocation was studied in a modified Ussing chamber using Escherichia coli K100. The quantity of mucus produced, as well as the incidence and quantity of bacteria translocating was measured. In the clonidine-treated animals, there was a significant decrease in the amount of mucus produced compared to the control animals. The clonidine animals also had a higher quantity of bacteria translocating during the reoxygenation period compared to the control animals. The mucus gel layer is protective against in vitro translocation of bacteria following an ischemia/reoxygenation insult.
黏液凝胶层被认为是肠道黏膜屏障的重要组成部分。本研究的目的是确定在肠黏膜缺血再灌注后减少黏液生成是否会改变体外大鼠黏膜模型中的细菌移位。可乐定用于减少与缺血/再灌注损伤相关的黏液生成。使用大肠杆菌K100在改良的尤斯灌流小室中研究细菌移位。测量所产生的黏液量以及细菌移位的发生率和数量。与对照动物相比，可乐定处理的动物所产生的黏液量显著减少。与对照动物相比，可乐定处理的动物在再氧合期间细菌移位的数量也更高。黏液凝胶层可保护免受缺血/再灌注损伤后细菌的体外移位。
