Menon K M, Gunaga K P
Neuroendocrinology. 1976;22(1):8-17. doi: 10.1159/000122608.
S.c. introduction of silastic capsules containing estradiol-17beta (E2) in the ovariectomized rat causes a surge of luteinizing hormone (LH) on the following day. The levels of cytoplasmic and nuclear E2 receptor complex were measured in the pituitary and hypothalamus at 0, 9, 24 and 33 h intervals after placing the E2 implant. The pituitary sensitivity to exogenous gonadotropin releasing hormone (GnRH) with respect to LH release in vitro was also measured. The results suggest that: (1) the pituitary sensitivity to exogenous GnRH remained higher 9, 24, and 33 h after placing the implant; (2) the circulating LH level decreased at 24 h and then increased at 33 h; (3) the pituitary and hypothalamic E2 receptor levels show striking differences during E2-induced LH release. Thus, the relationship between pituitary sensitivity to exogenous GnRH and the E2 receptor level in the pituitary and hypothalamus is complex.
给卵巢切除的大鼠皮下植入含17β-雌二醇（E2）的硅胶囊，会在次日引起促黄体生成素（LH）激增。在植入E2后，每隔0、9、24和33小时测量垂体和下丘脑中细胞质和细胞核E2受体复合物的水平。还测量了垂体在体外对促性腺激素释放激素（GnRH）刺激LH释放的敏感性。结果表明：（1）植入后9、24和33小时，垂体对外源GnRH的敏感性仍然较高；（2）循环LH水平在24小时时下降，然后在33小时时升高；（3）在E2诱导LH释放过程中，垂体和下丘脑的E2受体水平存在显著差异。因此，垂体对外源GnRH的敏感性与垂体和下丘脑中E2受体水平之间的关系很复杂。
