Norberg A N, Serra-Freire N M
Instituto de Biologia do Exército, Rua Francisco Manuel, Rio de Janeiro, Brasil.
Rev Inst Med Trop Sao Paulo. 1993 Nov-Dec;35(6):527-33.
The use of nuclear power through radiation for the destruction of microorganisms which cause food decay, and toxicosis, is specifically for peaceful purposes. Penicillium citrinum is a fungus which produce mycotoxins responsible for intoxication in humans and animals as a result of eating contaminated food. There is little informations on the resistance of P. citrinum to radiation. The objective of this research is to determine the lethal dose of gama radiation for these microorganisms. Seventy six suspensions containing approximately 100,000 spores/ml received a dose of radiation between 0.2 and 2.2 KGy (KiloGray), being one sample still alive re-irradiated with doses up to 3.0 KGy. The fungus were totally destroyed with a 2.2 KGy. Seventy six suspensions containing approximately 100,000 spores/ml received a dose of radiation between 0.2 and 2.2 KGy, being one sample still alive re-irradiated with doses up to 3.0 KGy. The fungus were totally destroyed with a 2.2 KGy dose. An increase in the resistance to lower dose levels of radiation was observed, in relation to the fungus which had not received irradiation.
the Minimum Lethal Dose (MLD) of gamma irradiation, for P. citrinum is 2.2 KGy; the re-irradiation of the surviving fungus demonstrate that occur appearance of radio-resistant mutants.
利用核能通过辐射来消灭导致食物腐烂和中毒的微生物，这一用途专门用于和平目的。桔青霉是一种真菌，它会产生霉菌毒素，导致人类和动物因食用受污染食物而中毒。关于桔青霉对辐射的抗性，相关信息较少。本研究的目的是确定这些微生物的伽马辐射致死剂量。七十六个含有约100,000个孢子/毫升的悬浮液接受了0.2至2.2千戈瑞（千格雷）的辐射剂量，其中一个仍存活的样本接受了高达3.0千戈瑞的再辐射。该真菌在2.2千戈瑞的剂量下被完全消灭。七十六个含有约100,000个孢子/毫升的悬浮液接受了0.2至2.2千戈瑞的辐射剂量，其中一个仍存活的样本接受了高达3.0千戈瑞的再辐射。该真菌在2.2千戈瑞的剂量下被完全消灭。与未接受辐射的真菌相比，观察到其对较低剂量辐射的抗性有所增加。
桔青霉伽马辐射的最小致死剂量（MLD）为2.2千戈瑞；对存活真菌的再辐射表明出现了抗辐射突变体。
