Malsch R, Harenberg J
1st Department of Medicine, Medical University Clinic Mannheim, Germany.
Semin Thromb Hemost. 1994;20(2):135-43. doi: 10.1055/s-2007-1001896.
The microheterogeneity of glycosaminoglycans was analyzed using HPSEC and PAGE. GAG preparations were analyzed five times to determine the standard deviation of the average molecular mass and the polydispersity P of each GAG with HPSEC. In addition, the Kav coefficient, the area under the absorbance time curve, and the peak purity were determined as mean and standard deviation. PAGE was performed five times using the same preparations and the average molecular mass and polydispersity were calculated. The results of PAGE and HPSEC were compared and the molecular mass distribution of the two methods was shown. The advantage of PAGE is the higher sensitivity and the resolution of oligosaccharides. The advantage of HPSEC is the better standardization, higher reproducibility, and speed.
使用高效体积排阻色谱法（HPSEC）和聚丙烯酰胺凝胶电泳（PAGE）分析糖胺聚糖的微观不均一性。对糖胺聚糖制剂进行了五次分析，以确定每种糖胺聚糖通过HPSEC测得的平均分子量的标准偏差和多分散性P。此外，测定了分配系数（Kav）、吸光度时间曲线下的面积和峰纯度的平均值和标准偏差。使用相同制剂进行五次PAGE实验，并计算平均分子量和多分散性。比较了PAGE和HPSEC的结果，并展示了两种方法的分子量分布。PAGE的优点是灵敏度更高，对寡糖的分辨率更高。HPSEC的优点是标准化更好、重现性更高且速度更快。
