Hale G L, Green T J
Department of Veterinary Microbiology, College of Veterinary Medicine, University of Missouri, Columbia 65211.
Am J Vet Res. 1994 Oct;55(10):1421-7.
An antigen extract of Dictyocaulus viviparus was analyzed by use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the antigen-recognition patterns of serum antibody from cattle not infected, cattle infected with D viviparus, and cattle with unknown history of D viviparus were analyzed by the use of ELISA and western blotting techniques. Cross-reactive antibody-recognition patterns were determined by comparing western blots of D viviparus-positive sera with blots of D viviparus-negative sera obtained from cattle singly infected with Bunostomum phlebotomum, Cooperia oncophora, C punctata, Nematodirus helvetianus, Oesophagostomum radiatum, or Ostertagia ostertagi. Five antigen bands unique to D viviparus were identified, and their frequency of appearance in western blots of sera from verified D viviparus-positive and -negative cattle, and sera from cattle exposed to the parasite, but with unknown D viviparus immune status, were determined. Of the 5 antigens unique to D viviparus, 29- and 19-kd bands had the highest frequencies of reaction (45.9 and 59.0%, respectively) with the test sera. These bands had strong reactivity with sera containing antibodies to D viviparus and did not react with the heterologous sera. We conclude that the 29- and 19-kd antigens may be useful for developing an improved serodiagnostic test for D viviparus infections in cattle.
使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对胎生网尾线虫的抗原提取物进行分析，并运用酶联免疫吸附测定法（ELISA）和蛋白质印迹技术，分析未感染牛、感染胎生网尾线虫的牛以及胎生网尾线虫感染史不明的牛血清抗体的抗原识别模式。通过比较胎生网尾线虫阳性血清与单独感染牛血矛线虫、牛环形泰勒虫、点状泰勒虫、瑞士细颈线虫、辐射食道口线虫或奥氏奥斯特线虫的牛的胎生网尾线虫阴性血清的蛋白质印迹，确定交叉反应抗体识别模式。鉴定出了胎生网尾线虫特有的五条抗原带，并测定了它们在经证实的胎生网尾线虫阳性和阴性牛血清以及接触过该寄生虫但胎生网尾线虫免疫状态不明的牛血清蛋白质印迹中的出现频率。在胎生网尾线虫特有的5种抗原中，29 kDa和19 kDa条带与测试血清的反应频率最高（分别为45.9%和59.0%）。这些条带与含有抗胎生网尾线虫抗体的血清有强烈反应，而与异源血清无反应。我们得出结论，29 kDa和19 kDa抗原可能有助于开发一种改进的牛胎生网尾线虫感染血清诊断试验。
