黑猩猩泡沫病毒分离株（SFV-6）长末端重复序列的结构与功能

Structure and function of the long terminal repeat of the chimpanzee foamy virus isolates (SFV-6).

作者信息

De Celis J, Tobaly-Tapiero J, Hampe A, Emanoil-Ravier R

机构信息

Laboratoire des Rétrovirus et Rétrotransposons des Vertébrés, UPR 0043 CNRS, Hopital Saint-Louis, Paris, France.

出版信息

Arch Virol. 1994;138(3-4):345-55. doi: 10.1007/BF01379137.

DOI:10.1007/BF01379137

PMID:7998839

Abstract

The complete long terminal repeat (LTR) nucleotide sequence of the chimpanzee foamy virus isolate SFV-6 was determined. Its 1761-bp size makes it the longest LTR reported to date among all retroviruses. Since the length of its LTR is similar to that of other simian isolates while its sequence homology is closer to that of HFV, SFV-6 genetic structure appears to be intermediate between simian and human foamy viruses. Transient expression assays demonstrate that SFV-6 encodes a transactivator of viral gene expression directed either by its own LTR or by heterologous promoters like HFV and HIV-1 LTRs. Our data also provide evidence for cross-transactivation between SFV-6 and HFV.

摘要

测定了黑猩猩泡沫病毒分离株SFV-6的完整长末端重复序列（LTR）。其1761个碱基对的大小使其成为迄今为止所有逆转录病毒中报道的最长LTR。由于其LTR的长度与其他猿猴分离株相似，而其序列同源性更接近HFV，SFV-6的遗传结构似乎介于猿猴泡沫病毒和人泡沫病毒之间。瞬时表达分析表明，SFV-6编码一种病毒基因表达反式激活因子，该因子可由其自身的LTR或由异源启动子（如HFV和HIV-1 LTR）指导。我们的数据还为SFV-6和HFV之间的交叉反式激活提供了证据。