Knee R S, Pitcher S E, Murphy P R
Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia, Canada.
Biochem Biophys Res Commun. 1994 Nov 30;205(1):577-83. doi: 10.1006/bbrc.1994.2704.
Basic fibroblast growth factor (bFGF) is a highly conserved and ubiquitously distributed mitogen. In amphibian oocytes bFGF mRNA is regulated post-transcriptionally by interaction with an antisense RNA transcript. We used reverse transcription-polymerase chain reaction (RT-PCR) and Northern hybridization to determine the presence of bFGF and its antisense RNA (gfg) in unfertilized human oocytes and postnatal differentiated tissues. BFGF and gfg transcripts were co-expressed in many tissues, with bFGF transcripts (7, 3.7 and 1.8 kb) being more abundant than the gfg transcript (1.5 kb) in 8 of 16 tissues examined. Sense and antisense expression was approximately equal in kidney and colon, while in heart, liver, skeletal muscle and testis gfg transcripts predominated. RT-PCR demonstrated the presence of bFGF and gfg transcripts in unfertilized oocytes where the antisense transcript was present in excess of the sense transcript. These findings suggest a role for gfg in regulation of bFGF expression.
碱性成纤维细胞生长因子（bFGF）是一种高度保守且广泛分布的促有丝分裂原。在两栖类卵母细胞中，bFGF mRNA通过与反义RNA转录本相互作用进行转录后调控。我们运用逆转录-聚合酶链反应（RT-PCR）和Northern杂交技术，来确定未受精的人类卵母细胞及出生后分化组织中bFGF及其反义RNA（gfg）的存在情况。在许多组织中，bFGF和gfg转录本共同表达，在所检测的16种组织中的8种里，bFGF转录本（7kb、3.7kb和1.8kb）比gfg转录本（1.5kb）更为丰富。在肾脏和结肠中，正义链和反义链的表达大致相等，而在心脏、肝脏、骨骼肌和睾丸中，gfg转录本占主导地位。RT-PCR证实未受精的卵母细胞中存在bFGF和gfg转录本，其中反义转录本的含量超过正义转录本。这些发现表明gfg在bFGF表达调控中发挥作用。
