Belbasi Masomeh, Jorsaraei Seyed Gholam Ali, Gholamitabar Tabari Maryam, Khanbabaei Ramzan
Department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran.
Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Science, Babol, Iran.
Cell J. 2017 Oct;19(3):476-481. doi: 10.22074/cellj.2017.3866. Epub 2017 Aug 19.
The aim of this study was to evaluate the fetal mouse lung tissue co-culture on in vitro maturation (IVM) of mouse immature oocytes.
In this experimental study, germinal vesicle (GV) oocytes from ovaries of a group of 25 female mice, 6-8 weeks of age, were dissected after being stimulated by 7.5 IU pregnant mare serum gonadotropin (PMSG) through an intraperitoneal (IP) injection. The fetal lung tissues were then prepared and cultured individually. A total number of 300 oocytes were cultured in the following three groups for 24 hours: control group (n=100) containing only base medium, group I (n=100) containing base medium co-cultured with 11.5- to 12.5-day old fetal mouse lung tissues, and group II (n=100) containing base medium co-cultured with 12.5- to 13.5-day old fetal mouse lung tissues. The proportion of GV and metaphase І (MI) oocytes matured into MІІ oocytes were compared among the three groups using analysis of variance (ANOVA). Correlation test were also used to evaluate the successful rate of IVM oocytes.
The proportions of GV oocytes reaching MІІ stage were 46, 65, and 56%, in control, I and II groups, respectively (P<0.05). The percentage of the oocytes remaining at the GV stage were higher in control group as compared with two treatment groups (P<0.05).
This study indicated that fetal mouse lung tissue co-culture method increased the percentage of GV oocytes reaching MII stage.
本研究旨在评估胎鼠肺组织共培养对小鼠未成熟卵母细胞体外成熟（IVM）的影响。
在本实验研究中，对一组25只6 - 8周龄雌性小鼠的卵巢进行解剖，通过腹腔注射7.5 IU孕马血清促性腺激素（PMSG）刺激后获取生发泡（GV）期卵母细胞。然后制备胎肺组织并单独培养。将总共300个卵母细胞分为以下三组培养24小时：对照组（n = 100）仅含基础培养基，第一组（n = 100）含基础培养基并与11.5至12.5日龄的胎鼠肺组织共培养，第二组（n = 100）含基础培养基并与12.5至13.5日龄的胎鼠肺组织共培养。使用方差分析（ANOVA）比较三组中从GV期和中期Ⅰ（MI）期成熟为MⅡ期的卵母细胞比例。还使用相关性检验来评估IVM卵母细胞的成功率。
对照组、第一组和第二组中达到MⅡ期的GV期卵母细胞比例分别为46%、65%和56%（P<0.05）。与两个处理组相比，对照组中停留在GV期的卵母细胞百分比更高（P<0.05）。
本研究表明胎鼠肺组织共培养方法提高了GV期卵母细胞达到MII期的百分比。
