Brulez H F, Heezius E C, de Fijter C W, Oe L P, Verhoef J, Verbrugh H A
Department of Nephrology, Free University Hospital Amsterdam, The Netherlands.
Adv Perit Dial. 1994;10:241-4.
The effects of a recently introduced peritoneal dialysis fluid (PDF) containing amino acids (AA) were compared with those of a glucose-based PDF (G-PDF) on viability and function of donor granulocytes (PMNs) in vitro. After 30 min incubation in the PDF, viability, assessed by trypan blue exclusion, and phagocytosis capacity (PC), tested in two assays using a fluorescein and a 3H-labeled Staphylococcus epidermidis strain, were significantly better in AA-PDF than in G-PDF (p < 0.002 in the 3H-assay). Bactericidal activity was not different in the PDFs. If pH of G-PDF was adjusted from 5.2 to neutral, differences in PC disappeared. In AA-PDF, PMN chemiluminescence (CL) response was significantly higher than in G-PDF (p < 0.003). At neutral pH, however, PMNs showed a significant stronger CL-response in 1.36% G-PDF than in AA-PDF (p < 0.05). These data suggest that this AA-PDF has little detrimental effect on phagocyte viability and function. The improved compatibility over G-PDF in in vitro tests seems to be pH dependent. The reduced chemiluminescence response compared to 1.36% G-PDF with neutral pH is possibly due to quenching by (one of the) amino acids and osmolarity.
将一种最近推出的含氨基酸（AA）的腹膜透析液（PDF）与基于葡萄糖的PDF（G-PDF）在体外对供体粒细胞（PMN）的活力和功能的影响进行了比较。在PDF中孵育30分钟后，通过台盼蓝排斥法评估的活力以及使用荧光素和3H标记的表皮葡萄球菌菌株在两种试验中测试的吞噬能力（PC），在AA-PDF中显著优于G-PDF（在3H试验中p < 0.002）。两种PDF中的杀菌活性没有差异。如果将G-PDF的pH从5.2调整至中性，PC的差异消失。在AA-PDF中，PMN化学发光（CL）反应显著高于G-PDF（p < 0.003）。然而，在中性pH下，PMN在1.36% G-PDF中的CL反应比在AA-PDF中显著更强（p < 0.05）。这些数据表明这种AA-PDF对吞噬细胞的活力和功能几乎没有有害影响。在体外试验中与G-PDF相比，其改善的兼容性似乎取决于pH。与具有中性pH的1.36% G-PDF相比，化学发光反应降低可能是由于氨基酸（其中之一）和渗透压的淬灭作用。
