Schricker T, Albuszies G, Kugler B, Wachter U, Georgieff M
Clinic of Anesthesiology, Ulm University, Germany.
Nutrition. 1994 Jul-Aug;10(4):342-5.
The stable isotope-labeled [1,1,2,3,3-2H5]glycerol analyzed by negative-ion chemical ionization (NCI) mass spectrometry has been proven a valid tracer for studying glycerol kinetics in humans. Because of its high technical complexity, NCI mass spectrometry is available to only a few laboratories. Thus, the aim of our study was to create an alternative method for measuring [1,1,2,3,3-2H5]glycerol enrichment in plasma with a new derivative and positive-ion chemical ionization (PCI) mass spectrometry. It could be demonstrated that the trisacetyl[1,1,2,3,3-2H5]glycerol derivative was able to produce a fragment at m/z = 164 with sufficient intensity. Application of [1,1,2,3,3-2H5]glycerol in seven healthy volunteers resulted in reproducible measurements of basal glycerol turnover rates. The mean glycerol flux rate of 3.02 +/- 0.37 mumol.kg-1 body wt.min-1 after an overnight fast was similar to values reported from studies with comparable protocols. Physiological changes of lipolysis rates after 48 h of fasting followed by infusion of 4 mg.kg-1 body wt.min-1 glucose could also be adequately studied in one subject. Fast-induced elevated glycerol turnover at 7.56 mumol.kg-1 body wt.min-1, was substantially suppressed to 1.13 mumol.kg-1 body wt.min-1, when glucose was administered. The easily performed trisacetyl[1,1,2,3,3-2H5]glycerol derivative analyzed by PCI mass spectrometry is suitable for studying glycerol kinetics in humans.
经负离子化学电离（NCI）质谱分析的稳定同位素标记的[1,1,2,3,3-2H5]甘油已被证明是研究人体甘油动力学的有效示踪剂。由于其技术复杂性高，只有少数实验室能够使用NCI质谱。因此，我们研究的目的是创建一种使用新衍生物和正离子化学电离（PCI）质谱测量血浆中[1,1,2,3,3-2H5]甘油富集的替代方法。可以证明，三乙酰基[1,1,2,3,3-2H5]甘油衍生物能够产生强度足够的m/z = 164处的碎片。在7名健康志愿者中应用[1,1,2,3,3-2H5]甘油可对基础甘油周转率进行可重复测量。过夜禁食后，甘油通量率平均为3.02±0.37 μmol·kg-1体重·min-1，与采用类似方案的研究报告的值相似。在一名受试者中也能够充分研究禁食48小时后接着输注4 mg·kg-1体重·min-1葡萄糖后脂解率的生理变化。禁食诱导的甘油周转率升高至7.56 μmol·kg-1体重·min-1，在给予葡萄糖后大幅降至1.13 μmol·kg-1体重·min-1。经PCI质谱分析的易于操作的三乙酰基[1,1,2,3,3-2H5]甘油衍生物适用于研究人体甘油动力学。
