用于区分海洋弧菌属菌种的M13 DNA指纹分析
M13 DNA fingerprinting in differentiation of marine Vibrio species.
作者信息
Chikarmane H, Bullis R A, Halvorson H O
机构信息
Marine Biological Laboratory, Woods Hole, Massachusetts 02543.
出版信息
Mol Mar Biol Biotechnol. 1994 Aug;3(4):200-5.
The identification and differentiation of bacterial strains and species are frequently carried out by the use of diagnostic biochemical profiles, serology, and the detection of restriction fragment length polymorphisms in genomic DNA. We show here that DNA restriction fragment length polymorphisms detected using a probe derived from bacteriophage M13 can discriminate between several marine Vibrio species. We have also demonstrated that individual isolates of Vibrio species can be differentiated using the M13 probe.
细菌菌株和种类的鉴定与区分通常通过使用诊断生化图谱、血清学以及检测基因组DNA中的限制性片段长度多态性来进行。我们在此表明，使用源自噬菌体M13的探针检测到的DNA限制性片段长度多态性能够区分几种海洋弧菌属菌种。我们还证明了，利用M13探针可以区分弧菌属菌种的各个分离株。
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