Seip J E, Fager S K, Gavagan J E, Anton D L, Di Cosimo R
Central Research and Development Department, E. I. du Pont de Nemours & Co., Wilmington, DE 19880-0328.
Bioorg Med Chem. 1994 Jun;2(6):371-8. doi: 10.1016/0968-0896(94)80003-0.
A variety of methods for the immobilization of glycolate oxidase have been examined for the preparation of a catalyst for the oxidation of glycolic acid to glyoxylic acid. The co-immobilization of glycolate oxidase and catalase on oxirane acrylic beads produced a catalyst which was stable to the reaction conditions used for the oxidation, where glycolic acid and oxygen are reacted in aqueous solution in the presence of the immobilized enzyme catalyst and ethylenediamine. Under optimum reaction conditions, 99% yields of glyoxylic acid were obtained at greater than 99% conversion of glycolic acid, and the recovery and reuse of the co-immobilized enzyme catalyst was demonstrated.
为制备用于将乙醇酸氧化为乙醛酸的催化剂，已对多种固定乙醇酸氧化酶的方法进行了研究。将乙醇酸氧化酶和过氧化氢酶共固定在环氧乙烷丙烯酸珠上，制得一种催化剂，该催化剂对用于氧化反应的条件具有稳定性，在固定化酶催化剂和乙二胺存在下，乙醇酸和氧气在水溶液中发生反应。在最佳反应条件下，乙醇酸转化率大于99%时，乙醛酸产率可达99%，且证明了共固定化酶催化剂可回收再利用。
