Dunipace A J, Zhang W, Beiswanger A J, Stookey G K
Indiana University School of Dentistry, Oral Health Research Institute, Indianapolis 46202.
Caries Res. 1994;28(5):315-21. doi: 10.1159/000261995.
This investigation tested and validated an in vitro model for studying the effects of fluoride on human dentin mineral content and fluoride uptake. Four studies examined the effects of different concentrations of fluoride, established dose-response profiles with NaF and Na2PO3F, and tested commercial dentifrices. A 7- or 14-day cyclic treatment regimen involved four 1-min exposures of sound human dentin specimens to the treatment agents, a 4-hour acid challenge period, and 20 h in human saliva daily. Mineral content was analyzed by image analysis microradiography and fluoride assays were performed using a microdrill biopsy technique. Data from these studies established the ability of the in vitro model to provide reproducible results, to demonstrate significant dose-related differences in the effects of both NaF- and Na2PO3F-containing treatments on dentin fluoride uptake and demineralization, and to detect a fluoride-induced reduction in dentin caries, relative to a nonfluoride control, similar to results established in a clinical trial.
本研究测试并验证了一种体外模型，用于研究氟化物对人牙本质矿物质含量和氟摄取的影响。四项研究考察了不同浓度氟化物的作用，建立了氟化钠（NaF）和氟磷酸钠（Na2PO3F）的剂量反应曲线，并对市售牙膏进行了测试。一种为期7天或14天的循环治疗方案包括将完好的人牙本质标本四次暴露于治疗剂中，每次暴露1分钟，接着是4小时的酸蚀挑战期，以及每天在人唾液中浸泡20小时。通过图像分析显微放射照相术分析矿物质含量，并使用微钻活检技术进行氟化物测定。这些研究的数据证实了该体外模型能够提供可重复的结果，能够证明含NaF和含Na2PO3F的治疗对牙本质氟摄取和脱矿作用存在显著的剂量相关差异，并且相对于无氟对照，能够检测到氟化物诱导的牙本质龋减少，这与临床试验得出的结果相似。
