Corkery D M, O'Connor K E, Buckley C M, Dobson A D
Food Microbiology Department, University College Cork, Ireland.
FEMS Microbiol Lett. 1994 Nov 15;124(1):23-7. doi: 10.1111/j.1574-6968.1994.tb07256.x.
Pseudomonas fluorescens strain CA-4 is a bioreactor isolate capable of ethylbenzene degradation. Transposon mutagenesis and enzyme assays have been performed which allow us to propose the ethylbenzene degradative pathway in operation in this strain. Ethylbenzene is initially converted to 2-phenylethanol. This is degraded to phenylacetaldehyde and then to phenylacetic acid. The major inducer of the pathway is ethylbenzene itself. The pathway is regulated by the presence of non-aromatic carbon sources. Oxidation of ethylbenzene is repressed by glutamate, but not by citrate or glucose. A clone from a chromosomal library has been found to complement a mutant deficient in the ability to convert ethylbenzene to 2-phenylethanol.
荧光假单胞菌CA-4菌株是一种能够降解乙苯的生物反应器分离株。已进行转座子诱变和酶分析，这使我们能够提出该菌株中运行的乙苯降解途径。乙苯最初转化为2-苯乙醇。2-苯乙醇降解为苯乙醛，然后再降解为苯乙酸。该途径的主要诱导剂是乙苯本身。该途径受非芳香族碳源的存在调控。乙苯的氧化受到谷氨酸的抑制，但不受柠檬酸盐或葡萄糖的抑制。已发现来自染色体文库的一个克隆能够互补缺乏将乙苯转化为2-苯乙醇能力的突变体。
