Moreno M A, Pascual C, Gibello A, Ferrer S, Bos C J, Debets A J, Suárez G
Department de Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
FEMS Microbiol Lett. 1994 Nov 15;124(1):35-41. doi: 10.1111/j.1574-6968.1994.tb07258.x.
A genetic transformation system for the aflatoxin-producing fungus Aspergillus parasiticus using two autonomously replicating plasmids from A. nidulans (ARp1 and pDHG25) is reported. Transformation frequencies using the plasmid pDHG25 were from 5 x 10(2) to 2.5 x 10(4) transformants per 10(6) viable protoplasts and microgram DNA. The stability of the plasmids in the transformants was also studied. This transformation system offers a new opportunity to clone genes related to aflatoxin production using appropriate aflatoxin-defective mutants.
报道了一种利用来自构巢曲霉的两个自主复制质粒（ARp1和pDHG25）构建产黄曲霉毒素寄生曲霉的遗传转化系统。使用质粒pDHG25的转化频率为每10^6个活原生质体和微克DNA产生5×10^2至2.5×10^4个转化体。还研究了质粒在转化体中的稳定性。该转化系统为利用合适的黄曲霉毒素缺陷型突变体克隆与黄曲霉毒素产生相关的基因提供了新机会。
