Chaturvedi V P, Vanegas R, Chaffin W L
Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock 79430.
FEMS Microbiol Lett. 1994 Nov 15;124(1):99-105. doi: 10.1111/j.1574-6968.1994.tb07268.x.
If the determinants of shape and cell wall topography are independently regulated and induced in germ tube formation in Candida albicans, these processes may be separable in a non-germ tube forming strain. The expression of several preferentially expressed hyphal surface components in a parental, non-germ tube forming variant, and a germ tube forming revertant strain were examined by indirect immunofluorescence. The proportion of germ tubes expressing the determinants and the morphological localization of expression was similar. Few yeast cells in germ tube cultures bound probes and there was no increase in binding by yeast cells of the variant strain. Extraction with beta-mercaptoethanol prior to analysis had little effect on probe binding and the shape of yeast cells were similar. These observations suggest the ability to promote apical expansion in germ tube formation and surface expression of certain markers were coordinately regulated.
如果白色念珠菌芽管形成过程中形状和细胞壁拓扑结构的决定因素是独立调控和诱导的，那么在非芽管形成菌株中这些过程可能是可分离的。通过间接免疫荧光检查了亲本非芽管形成变体和芽管形成回复菌株中几种优先表达的菌丝表面成分的表达情况。表达决定因素的芽管比例和表达的形态定位相似。芽管培养物中很少有酵母细胞结合探针，变体菌株的酵母细胞结合也没有增加。分析前用β-巯基乙醇提取对探针结合影响很小，酵母细胞的形状相似。这些观察结果表明，芽管形成过程中促进顶端扩张的能力和某些标记物的表面表达是协同调控的。
