Bisht K S, Revathi C J, Srinivas U K
Centre for Cellular and Molecular Biology, Hyderabad, India.
Indian J Biochem Biophys. 1994 Aug;31(4):295-301.
Heat shock to embryonal carcinoma cells PCC4 at 45 degrees C for 30 min resulted in the differentiation of cells although heat shock response was induced on exposure to 42 degrees C for 60 min. Differentiated cells were large and well spread with reduced nuclear/cytoplasmic ratios as compared to undifferentiated cells. Change in cell morphology was associated with the disappearance and appearance of stage specific embryonic antigens 1 and 3 respectively. We also found a change in intracellular pH in PCC4 cells within 30 min of heat shock as measured by the change in fluorescence intensity of a probe incorporated into cells during heat shock.
将胚胎癌细胞PCC4在45摄氏度下热休克30分钟可导致细胞分化，尽管在42摄氏度下暴露60分钟会诱导热休克反应。与未分化细胞相比，分化后的细胞体积较大且铺展良好，核质比降低。细胞形态的变化分别与阶段特异性胚胎抗原1和3的消失和出现相关。我们还发现，通过热休克期间掺入细胞的探针荧光强度变化测量，热休克30分钟内PCC4细胞的细胞内pH发生了变化。
