Karim B, Béliard R, Huart J J, Bourel D
Centre Régional de Transfusion Sanguine, Lille, France.
Immunol Lett. 1994 Jul;41(2-3):139-45. doi: 10.1016/0165-2478(94)90124-4.
Eight murine anti-TNF alpha monoclonal antibodies (mAb) were produced after immunization of BALB/c mice with rhTNF alpha. Six of these mAbs were able to neutralize cytotoxic activity of TNF alpha against L929 cells. Two other mAbs had no neutralizing effect. Epitope mapping studies were performed by ELISA and by using a BIAcore system (Pharmacia). The described mAbs were allowed to define 4 different epitopes on TNF alpha. Three of them were involved in the binding of TNF alpha with its receptor (cytotoxic neutralization of TNF alpha). Another epitope was defined by non-neutralizing mAbs.
用重组人肿瘤坏死因子α（rhTNFα）免疫BALB/c小鼠后产生了8种鼠抗TNFα单克隆抗体（mAb）。其中6种mAb能够中和TNFα对L929细胞的细胞毒活性。另外2种mAb没有中和作用。通过酶联免疫吸附测定（ELISA）和使用BIAcore系统（Pharmacia公司）进行了表位作图研究。所描述的mAb能够确定TNFα上4个不同的表位。其中3个表位参与TNFα与其受体的结合（TNFα的细胞毒中和作用）。另一个表位由非中和性mAb确定。
