Kilim Y, Rosenblatt J D, Danon Y L
Kipper Institute of Immunology, Felsenstein Research Center, Children's Medical Center of Israel, Petah Tikva.
Isr J Med Sci. 1994 Dec;30(12):891-4.
Recent studies have established the presence of human T cell leukemia/lymphoma virus type I (HTLV-I) in Israel. The entire nucleotide sequence of HTLV-I virus from a previously described HE isolate of a Mashadi Jewish Iranian patient was determined. To further characterize the LTR and env genes from the HTLV-I isolate we employed polymerase chain reaction amplification with subsequent cloning and sequencing of the amplified products on both strands. Sequence analyses of amplified LTR regions of this variant showed marked nucleotide homology of 98% compared to Japanese isolates, while African and Indo-Malay (Papua, New Guinea) and Solomon Island isolates showed more divergence with sequence homology of 95% and 91%. Higher homology of 98-99% was conserved in the amplified HTLV-env gene. In this respect the Iranian isolate was most similar to the African and Japanese isolate and divergent from the Melanesian HTLV-I variant, supporting the theory that HTLV-I may have originated in Africa and reached the Far East by overland trade routes.
最近的研究证实以色列存在人类T细胞白血病/淋巴瘤病毒I型（HTLV-I）。测定了一名来自伊朗马沙迪犹太患者的先前描述的HE分离株中HTLV-I病毒的完整核苷酸序列。为了进一步表征HTLV-I分离株的长末端重复序列（LTR）和包膜（env）基因，我们采用聚合酶链反应扩增，随后对扩增产物的两条链进行克隆和测序。对该变体扩增的LTR区域进行序列分析表明，与日本分离株相比，其核苷酸同源性高达98%，而非洲、印度-马来（巴布亚新几内亚）和所罗门群岛分离株的序列同源性分别为95%和91%，差异更大。扩增的HTLV-env基因保守性更高，同源性为98%-99%。在这方面，伊朗分离株与非洲和日本分离株最为相似，与美拉尼西亚HTLV-I变体不同，这支持了HTLV-I可能起源于非洲并通过陆路贸易路线到达远东的理论。
