Leong S P, Hornung M O, Krementz E T
Oncology. 1976;33(5-6):246-9. doi: 10.1159/000225156.
Eight chimpanzees were immunized, each with a single melanoma cell line. It was found that the serum of only one of the eight chimpanzees showed immunofluorescence with four of the five melanoma cell lines at the membrane surface; but it showed no fluorescence with normal skin fibroblasts, WI-38 cells, HeLa cells, human or monkey kidney cells. With appropriate absorption studies it was found that the antiserum was specific to melanoma cell lines. Trypsinated cells showed bright patchy membrane fluorescence, whereas non-trypsinated cells showed bright full ring fluorescence. The chimpanzee's antibody was C'-fixing IgG. It was concluded that the immunized chimpanzee produced antibody to surface antigen(s) common to four of the five melanoma cell lines tested.
八只黑猩猩接受了免疫，每只接种一种黑色素瘤细胞系。结果发现，八只黑猩猩中只有一只的血清与五种黑色素瘤细胞系中的四种在细胞膜表面呈现免疫荧光；但与正常皮肤成纤维细胞、WI - 38细胞、HeLa细胞、人或猴肾细胞均无荧光反应。通过适当的吸收研究发现，该抗血清对黑色素瘤细胞系具有特异性。经胰蛋白酶处理的细胞呈现明亮的斑片状膜荧光，而未经胰蛋白酶处理的细胞呈现明亮的完整环状荧光。黑猩猩的抗体是补体结合性IgG。得出的结论是，免疫的黑猩猩产生了针对所测试的五种黑色素瘤细胞系中四种共有的表面抗原的抗体。
