Schulze H, van Leeuwen P, Verstegen M W, Huisman J, Souffrant W B, Ahrens F
Department of Animal Nutrition, Agricultural University, Wageningen, The Netherlands.
J Anim Sci. 1994 Sep;72(9):2362-8. doi: 10.2527/1994.7292362x.
A study was conducted with 20 barrows (average BW 25 kg) to determine the effect of various levels of neutral detergent fiber (NDF) in the diet on the apparent ileal nutrient digestibility and ileal diaminopimelic acid (DAPA) content, and consequently on the amount of ileal endogenous nitrogen. The pigs were fitted with a post-valvular T-cecal cannula. They were fed .8 kg/d of a corn starch-based semisynthetic diet formulated to contain equal amounts of protein and starch and 0, 60, 120, or 180 g of purified NDF/kg of diet, included at the expense of glucose. The purified NDF (pNDF) was isolated from wheat bran using an incubation procedure with pancreatin. Ileal digestibility of NDF was approximately 17% and was independent of the pNDF level in the diet. By increasing the amount of pNDF in the diets, apparent ileal digestibilities of dry matter (DM), nitrogen (N), neutral detergent insoluble N (NDF-N), and ash decreased linearly (P < .05). The DAPA content of the ileal digesta (g/d) was not affected by the percentage of pNDF in the diets. Calculation of the ileal bacterial N excretion indicated that more than 50% of the ileal N was of bacterial origin. With increased percentage of dietary pNDF, both endogenous and exogenous N in ileal chyme were linearly increased (P < .05). Thus, an increase in the dietary fiber content leads to a decreased apparent ileal protein digestibility due to increased ileal losses of both endogenous and exogenous protein.
用20头公猪（平均体重25千克）进行了一项研究，以确定日粮中不同水平的中性洗涤纤维（NDF）对回肠表观养分消化率和回肠二氨基庚二酸（DAPA）含量的影响，进而确定回肠内源氮的量。给猪安装了回肠末端T型盲肠套管。给它们每天饲喂0.8千克以玉米淀粉为基础的半合成日粮，该日粮配方中蛋白质和淀粉含量相等，且每千克日粮含有0、60、120或180克纯化NDF，用葡萄糖替代其他成分。纯化NDF（pNDF）是通过用胰蛋白酶孵育从小麦麸皮中分离出来的。NDF的回肠消化率约为17%，且与日粮中pNDF水平无关。通过增加日粮中pNDF的量，干物质（DM）、氮（N）、中性洗涤不溶性氮（NDF-N）和灰分的回肠表观消化率呈线性下降（P<0.05）。日粮中pNDF的百分比不影响回肠食糜的DAPA含量（克/天）。回肠细菌氮排泄量的计算表明，回肠中超过50%的氮来自细菌。随着日粮中pNDF百分比的增加，回肠食糜中的内源氮和外源氮均呈线性增加（P<0.05）。因此，日粮纤维含量的增加会导致回肠表观蛋白质消化率降低，这是由于内源和外源蛋白质在回肠中的损失增加所致。
