Gao J, Sherman W M, McCune S A, Osei K
School of Health, Physical Education, and Recreation, Department of Food Science and Technology, Ohio State University, Columbus 43210.
J Appl Physiol (1985). 1994 Aug;77(2):534-41. doi: 10.1152/jappl.1994.77.2.534.
This study utilized the obese male spontaneously hypertensive heart failure rat (SHHF/Mcc-facp), which has metabolic features very similar to human non-insulin-dependent diabetes mellitus. The purpose of this study was to assess the insulin sensitivity and responsiveness of whole body glucose disposal and insulin suppressability of hepatic glucose production with use of the euglycemic-hyperinsulinemic clamp procedure in 12- to 15-wk-old SHHF/Mcc-facp rats at rest (OS) and 2.5 h after a single session of acute exercise (OE). Lean male SHHF/Mcc-facp rats were sedentary (LS) control animals. At least three clamps producing different insulin-stimulated responses were performed on each animal in a randomized order. At this age the obese animals are normotensive and have not developed congestive heart failure. Compared with LS, OS were significantly hyperglycemic and hyperinsulinemic and insulin sensitivity and responsiveness of whole body glucose uptake and insulin suppressability of hepatic glucose production were significantly decreased. Compared with LS and OS, acute exercise significantly decreased resting plasma glucose but did not alter plasma insulin. Compared with OS, acute exercise significantly increased the insulin responsiveness of whole body glucose disposal but did not affect the sensitivity of whole body glucose disposal or insulin suppressability of hepatic glucose production. Compared with LS, however, acute exercise did not "normalize" the insulin responsiveness of whole body glucose disposal. Thus a single acute exercise session improves but does not normalize whole body insulin resistance in the SHHF/Mcc-facp rat.
本研究使用了肥胖雄性自发性高血压心力衰竭大鼠（SHHF/Mcc-facp），其代谢特征与人类非胰岛素依赖型糖尿病非常相似。本研究的目的是通过正常血糖-高胰岛素钳夹技术，评估12至15周龄的SHHF/Mcc-facp大鼠在静息状态（OS）和单次急性运动2.5小时后（OE）的全身葡萄糖处置的胰岛素敏感性和反应性，以及肝脏葡萄糖生成的胰岛素抑制作用。瘦雄性SHHF/Mcc-facp大鼠作为久坐不动的（LS）对照动物。对每只动物随机进行至少三次产生不同胰岛素刺激反应的钳夹实验。在这个年龄段，肥胖动物血压正常，尚未发展为充血性心力衰竭。与LS组相比，OS组显著高血糖和高胰岛素血症，全身葡萄糖摄取的胰岛素敏感性和反应性以及肝脏葡萄糖生成的胰岛素抑制作用显著降低。与LS组和OS组相比，急性运动显著降低了静息血浆葡萄糖水平，但未改变血浆胰岛素水平。与OS组相比，急性运动显著提高了全身葡萄糖处置的胰岛素反应性，但不影响全身葡萄糖处置的敏感性或肝脏葡萄糖生成的胰岛素抑制作用。然而，与LS组相比，急性运动并未使全身葡萄糖处置的胰岛素反应性“正常化”。因此，单次急性运动可改善SHHF/Mcc-facp大鼠的全身胰岛素抵抗，但不能使其正常化。
