Maemura K, Kurihara H, Kurihara Y, Nagai R, Yazaki Y
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1994 Jun 15;201(2):538-45. doi: 10.1006/bbrc.1994.1735.
Microvascular endothelial cells were isolated from endothelin (ET)-1 knockout mice. The ET-1-lacking endothelial cells represent normal shape and activity of acetyl-LDL uptake. The growth of ET-1-lacking endothelial cells was not different from that of control cells in the presence of serum. Compensatory production of other ET isoforms does not occur in these cells. Conversion of big ET-1 to ET-1 is not different between ET-1-lacking and control cells. These results suggest that ET-1 is not essential to endothelial morphology and growth, that the expression of ET isoforms is not redundant in endothelial cells, and that the machinery processing ET-1 is preserved despite of the absence of its substrate.
从内皮素（ET）-1基因敲除小鼠中分离出微血管内皮细胞。缺乏ET-1的内皮细胞呈现出正常的形态以及乙酰化低密度脂蛋白摄取活性。在有血清存在的情况下，缺乏ET-1的内皮细胞的生长与对照细胞并无差异。这些细胞中不会发生其他ET异构体的代偿性产生。在缺乏ET-1的细胞和对照细胞之间，大ET-1向ET-1的转化没有差异。这些结果表明，ET-1对于内皮细胞形态和生长并非必不可少，ET异构体在内皮细胞中的表达并非多余，并且尽管缺乏底物，处理ET-1的机制仍然保留。
