Yamamoto H, Itoh F, Hinoda Y, Senota A, Yoshimoto M, Nakamura H, Imai K, Yachi A
First Department of Internal Medicine, Sapporo Medical University School of Medicine, Japan.
Biochem Biophys Res Commun. 1994 Jun 15;201(2):657-64. doi: 10.1006/bbrc.1994.1751.
Matrilysin is believed to have a role in tumor progression. Its expression correlates with the occurrence of colorectal cancer. We have examined the expression of matrilysin mRNA in various colorectal disorders and its localization using RT-PCR and in situ hybridization. We have also examined whether Matrilysin is induced by cell to matrix interaction. Matrilysin mRNA was detected in all adenoma tissues examined, whereas none was detectable in hyperplastic polyps, mildly inflamed regions of ulcerative colitis or normal colon tissues, and its message was localized in adenoma cells themselves. In addition, levels of enzyme activities of matrilysin were lower in adenomas compared with cancers in casein zymography. Matrilysin mRNA was induced by immobilized truncated fibronectin or RGD peptide. Thus, matrilysin may play an important role in colorectal carcinogenesis.
基质溶解素被认为在肿瘤进展中起作用。其表达与结直肠癌的发生相关。我们使用逆转录聚合酶链反应（RT-PCR）和原位杂交技术检测了基质溶解素mRNA在各种结直肠疾病中的表达及其定位。我们还研究了细胞与基质相互作用是否会诱导基质溶解素。在所检测的所有腺瘤组织中均检测到基质溶解素mRNA，而在增生性息肉、溃疡性结肠炎轻度炎症区域或正常结肠组织中均未检测到，其信号定位于腺瘤细胞本身。此外，在酪蛋白酶谱分析中，腺瘤中基质溶解素的酶活性水平低于癌组织。固定化的截短纤连蛋白或RGD肽可诱导基质溶解素mRNA表达。因此，基质溶解素可能在结直肠癌发生中起重要作用。
