Martínez-Ramírez A C, González-Nebauer S, Escriche B, Real M D
Department of Genetics, University of Valencia, Spain.
Biochem Biophys Res Commun. 1994 Jun 15;201(2):782-7. doi: 10.1006/bbrc.1994.1769.
The CryIA(a), CryIA(b) and CryIA(c) Bacillus thuringiensis insecticidal crystal proteins (ICPs) were used in ligand-blot experiments to detect specific binding proteins in brush-border membrane vesicles (BBMV) of Manduca sexta. We identified a protein which binds these three CryIA-type ICPs. The apparent molecular mass of the protein, estimated on SDS-PAGE, was 210 kDa as was the CryIA(b) binding protein previously described by Vadlamudi and col. We have also demonstrated, in ligand blot experiments, that CryIA(a) and CryIA(c) compete with CryIA(b) for binding this 210 kDa protein. Properties of the binding molecule can be correlated with knowledge previously acquired through radiolabelled binding experiments.
苏云金芽孢杆菌的CryIA(a)、CryIA(b)和CryIA(c)杀虫晶体蛋白（ICPs）用于配体印迹实验，以检测烟草天蛾中肠刷状缘膜囊泡（BBMV）中的特异性结合蛋白。我们鉴定出一种能结合这三种CryIA型ICPs的蛋白质。在SDS-PAGE上估计该蛋白质的表观分子量为210 kDa，与Vadlamudi等人先前描述的CryIA(b)结合蛋白相同。我们还在配体印迹实验中证明，CryIA(a)和CryIA(c)与CryIA(b)竞争结合这种210 kDa的蛋白质。结合分子的特性可以与先前通过放射性标记结合实验获得的知识相关联。
