• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Binding of Bacillus thuringiensis Cry1 Toxins to the Midgut Brush Border Membrane Vesicles of Chilo suppressalis (Lepidoptera: Pyralidae): Evidence of Shared Binding Sites.苏云金芽胞杆菌 Cry1 毒素与二化螟（鳞翅目：螟蛾科）中肠刷状缘膜囊泡的结合：结合位点的证据。
Appl Environ Microbiol. 1996 May;62(5):1544-9. doi: 10.1128/aem.62.5.1544-1549.1996.
2
Binding of Bacillus thuringiensis Cry1 Toxins to the Midgut Brush Border Membrane Vesicles of Chilo suppressalis (Lepidoptera: Pyralidae): Evidence of Shared Binding Sites.苏云金芽胞杆菌 Cry1 毒素与斜纹夜蛾（鳞翅目：夜蛾科）中肠刷状缘膜囊泡的结合：结合位点的证据。
Appl Environ Microbiol. 1996 Aug;62(8):3073. doi: 10.1128/aem.62.8.3073-3073b.1996.
3
Determination of Binding of Bacillus thuringiensis (delta)-Endotoxin Receptors to Rice Stem Borer Midguts.测定苏云金芽孢杆菌（δ）-内毒素受体与水稻螟虫中肠的结合。
Appl Environ Microbiol. 1997 Apr;63(4):1453-9. doi: 10.1128/aem.63.4.1453-1459.1997.
4
Bacillus thuringiensis delta-endotoxin binding to brush border membrane vesicles of rice stem borers.苏云金芽孢杆菌δ-内毒素与水稻螟虫刷状缘膜囊泡的结合
Arch Insect Biochem Physiol. 2004 Apr;55(4):169-77. doi: 10.1002/arch.10128.
5
Binding of Bacillus thuringiensis toxins in resistant and susceptible strains of pink bollworm (Pectinophora gossypiella).苏云金芽孢杆菌毒素在抗药性和敏感型棉铃虫（棉红铃虫）品系中的结合情况
Insect Biochem Mol Biol. 2003 Sep;33(9):929-35. doi: 10.1016/s0965-1748(03)00099-7.
6
Screen of Bacillus thuringiensis toxins for transgenic rice to control Sesamia inferens and Chilo suppressalis.筛选苏云金芽孢杆菌毒素用于转基因水稻以防治二化螟和稻纵卷叶螟。
J Invertebr Pathol. 2010 Sep;105(1):11-5. doi: 10.1016/j.jip.2010.05.002. Epub 2010 May 7.
7
Toxicity and receptor binding properties of Bacillus thuringiensis delta-endotoxins to the midgut brush border membrane vesicles of the rice leaf folders, Cnaphalocrocis medinalis and Marasmia patnalis.苏云金芽孢杆菌δ-内毒素对稻纵卷叶螟和显纹纵卷叶螟中肠刷状缘膜囊泡的毒性及受体结合特性
Curr Microbiol. 2000 Oct;41(4):276-83. doi: 10.1007/s002840010134.
8
Use of Bacillus thuringiensis toxins for control of the cotton pest Earias insulana (Boisd.) (Lepidoptera: Noctuidae).苏云金芽孢杆菌毒素用于防治棉花害虫埃及伊蚊夜蛾（鳞翅目：夜蛾科）。
Appl Environ Microbiol. 2006 Jan;72(1):437-42. doi: 10.1128/AEM.72.1.437-442.2006.
9
Exploring the midgut transcriptome and brush border membrane vesicle proteome of the rice stem borer, Chilo suppressalis (Walker).探索水稻螟虫（Chilo suppressalis （Walker））中肠转录组和刷状缘膜囊泡蛋白质组。
PLoS One. 2012;7(5):e38151. doi: 10.1371/journal.pone.0038151. Epub 2012 May 29.
10
Importance of Cry1 delta-endotoxin domain II loops for binding specificity in Heliothis virescens (L.).Cry1δ-内毒素结构域II环对烟芽夜蛾（L.）结合特异性的重要性。
Appl Environ Microbiol. 2001 Jan;67(1):323-9. doi: 10.1128/AEM.67.1.323-329.2001.

引用本文的文献

1
Effects of Bacillus thuringiensis var. israelensis on the Black Fly Communities (Diptera, Simuliidae) in Tropical Streams.苏云金芽孢杆菌以色列变种对热带溪流中黑蝇群落（双翅目，蚋科）的影响。
Neotrop Entomol. 2021 Apr;50(2):269-281. doi: 10.1007/s13744-020-00842-2. Epub 2021 Feb 10.
2
Bacillus thuringiensis Is an Environmental Pathogen and Host-Specificity Has Developed as an Adaptation to Human-Generated Ecological Niches.苏云金芽孢杆菌是一种环境病原体，宿主特异性的形成是对人类创造的生态位的一种适应。
Insects. 2013 Dec 24;5(1):62-91. doi: 10.3390/insects5010062.
3
Binding site concentration explains the differential susceptibility of Chilo suppressalis and Sesamia inferens to Cry1A-producing rice.结合位点浓度解释了二化螟和大螟对转Cry1A基因水稻的不同敏感性。
Appl Environ Microbiol. 2014 Aug;80(16):5134-40. doi: 10.1128/AEM.01544-14. Epub 2014 Jun 13.
4
Receptors and Lethal Effect of Bacillus thuringiensis Insecticidal Crystal Proteins to the Anticarsia gemmatalis (Lepidoptera, Noctuidae).苏云金芽孢杆菌杀虫晶体蛋白对豆夜蛾（鳞翅目，夜蛾科）的受体及致死效应
ISRN Microbiol. 2013 Sep 30;2013:940284. doi: 10.1155/2013/940284. eCollection 2013.
5
The effect of bacterial challenge on ferritin regulation in the yellow fever mosquito, Aedes aegypti.细菌挑战对黄热病蚊（埃及伊蚊）中铁蛋白调节的影响。
Insect Sci. 2013 Oct;20(5):601-19. doi: 10.1111/j.1744-7917.2012.01581.x. Epub 2012 Dec 11.
6
A Change in a Single Midgut Receptor in the Diamondback Moth (Plutella xylostella) Is Only in Part Responsible for Field Resistance to Bacillus thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai.小菜蛾（Plutella xylostella）中单个中肠受体的变化仅部分导致对苏云金芽孢杆菌亚种 kurstaki 和苏云金芽孢杆菌亚种 aizawai 的田间抗性。
Appl Environ Microbiol. 1997 May;63(5):1814-9. doi: 10.1128/aem.63.5.1814-1819.1997.
7
Determination of Binding of Bacillus thuringiensis (delta)-Endotoxin Receptors to Rice Stem Borer Midguts.测定苏云金芽孢杆菌（δ）-内毒素受体与水稻螟虫中肠的结合。
Appl Environ Microbiol. 1997 Apr;63(4):1453-9. doi: 10.1128/aem.63.4.1453-1459.1997.
8
Interaction between functional domains of Bacillus thuringiensis insecticidal crystal proteins.苏云金芽孢杆菌杀虫晶体蛋白功能结构域之间的相互作用。
Appl Environ Microbiol. 1999 Jul;65(7):2918-25. doi: 10.1128/AEM.65.7.2918-2925.1999.

本文引用的文献

1
Binding of Insecticidal Crystal Proteins of Bacillus thuringiensis to the Midgut Brush Border of the Cabbage Looper, Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae), and Selection for Resistance to One of the Crystal Proteins.苏云金芽孢杆菌杀虫晶体蛋白与斜纹夜蛾中肠刷状缘的结合及其对其中一种晶体蛋白的抗性选择
Appl Environ Microbiol. 1994 Oct;60(10):3840-6. doi: 10.1128/aem.60.10.3840-3846.1994.
2
Two Different Bacillus thuringiensis Delta-Endotoxin Receptors in the Midgut Brush Border Membrane of the European Corn Borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Pyralidae).两种不同的苏云金芽孢杆菌δ-内毒素受体在欧洲玉米螟中肠刷状缘膜中的表达（鳞翅目：夜蛾科）。
Appl Environ Microbiol. 1993 Jun;59(6):1828-37. doi: 10.1128/aem.59.6.1828-1837.1993.
3
[Amino acid-p-nitroanilide as a substrate for aminopeptidases and other proteolytic enzymes].[氨基酸对硝基苯胺作为氨肽酶和其他蛋白水解酶的底物]
Hoppe Seylers Z Physiol Chem. 1962 Nov 15;329:278-88. doi: 10.1515/bchm2.1962.329.1.278.
4
Broad-spectrum resistance to Bacillus thuringiensis toxins in Heliothis virescens.烟草天蛾对苏云金芽孢杆菌毒素的广谱抗性
Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):7986-90. doi: 10.1073/pnas.89.17.7986.
5
Ligand blot identification of a Manduca sexta midgut binding protein specific to three Bacillus thuringiensis CryIA-type ICPs.通过配体印迹法鉴定烟草天蛾中肠一种对三种苏云金芽孢杆菌CryIA型杀虫晶体蛋白具有特异性的结合蛋白。
Biochem Biophys Res Commun. 1994 Jun 15;201(2):782-7. doi: 10.1006/bbrc.1994.1769.
6
A comparison and analysis of the toxicity and receptor binding properties of Bacillus thuringiensis CryIC delta-endotoxin on Spodoptera littoralis and Bombyx mori.苏云金芽孢杆菌CryICδ-内毒素对斜纹夜蛾和家蚕的毒性及受体结合特性的比较与分析
FEBS Lett. 1994 Oct 24;353(3):259-63. doi: 10.1016/0014-5793(94)01057-9.
7
A new solid-state reagent to iodinate proteins. I. Conditions for the efficient labeling of antiserum.一种用于蛋白质碘化的新型固态试剂。I. 高效标记抗血清的条件。
Anal Biochem. 1982 Sep 15;125(2):427-32. doi: 10.1016/0003-2697(82)90025-2.
8
Ligand: a versatile computerized approach for characterization of ligand-binding systems.配体：一种用于表征配体结合系统的通用计算机化方法。
Anal Biochem. 1980 Sep 1;107(1):220-39. doi: 10.1016/0003-2697(80)90515-1.
9
Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta.苏云金芽孢杆菌库尔斯塔克亚种HD - 73晶体蛋白的全长和截短质粒克隆及其对烟草天蛾的毒性。
Gene. 1985;36(3):289-300. doi: 10.1016/0378-1119(85)90184-2.
10
Specificity of Bacillus thuringiensis delta-endotoxins is correlated with the presence of high-affinity binding sites in the brush border membrane of target insect midguts.苏云金芽孢杆菌δ-内毒素的特异性与目标昆虫中肠刷状缘膜中高亲和力结合位点的存在相关。
Proc Natl Acad Sci U S A. 1988 Nov;85(21):7844-8. doi: 10.1073/pnas.85.21.7844.

苏云金芽胞杆菌 Cry1 毒素与二化螟（鳞翅目：螟蛾科）中肠刷状缘膜囊泡的结合：结合位点的证据。

Binding of Bacillus thuringiensis Cry1 Toxins to the Midgut Brush Border Membrane Vesicles of Chilo suppressalis (Lepidoptera: Pyralidae): Evidence of Shared Binding Sites.

出版信息

Appl Environ Microbiol. 1996 May;62(5):1544-9. doi: 10.1128/aem.62.5.1544-1549.1996.

DOI:10.1128/aem.62.5.1544-1549.1996
PMID:16535306
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1388844/
Abstract

Binding and competition among Cry1Aa, Cry1Ac, and Cry1Ba toxins were analyzed quantitatively in vitro by using (sup125)I-labeled activated toxins and brush border membrane vesicles isolated from Chilo suppressalis larval midguts. The three toxins bound specifically to the midgut brush border membrane vesicles. Direct binding experiments showed that Cry1Aa and Cry1Ba recognized a single class of binding sites with different affinities, whereas Cry1Aa recognized two classes of binding sites, one with a high affinity and a low concentration and the other with a lower affinity but higher concentration. Competition experiments showed that toxins Cry1Ac and Cry1Ba shared a binding site in the C. suppressalis midgut membranes and that this site was also the low-affinity binding site for Cry1Aa.

摘要

采用（sup125）I 标记的激活毒素和从小菜蛾幼虫中肠分离的刷状缘膜泡，在体外对 Cry1Aa、Cry1Ac 和 Cry1Ba 毒素之间的结合和竞争进行了定量分析。三种毒素特异性结合到中肠刷状缘膜泡上。直接结合实验表明，Cry1Aa 和 Cry1Ba 识别具有不同亲和力的单一结合位点，而 Cry1Aa 识别两个结合位点，一个具有高亲和力和低浓度，另一个具有较低亲和力但较高浓度。竞争实验表明，Cry1Ac 和 Cry1Ba 毒素在小菜蛾中肠膜上共享一个结合位点，该位点也是 Cry1Aa 的低亲和力结合位点。