Holmberg E G, Reuer Q R, Geisert E E, Owens J L
Department of Chemistry/Physics, University of Alaska Anchorage 99508.
Biochem Biophys Res Commun. 1994 Jun 15;201(2):888-93. doi: 10.1006/bbrc.1994.1785.
Immunoliposomes were constructed with an antibody specific to glial cells. They were used to examine the specificity and efficacy of cell type plasmid transfection. Liposomes contained a beta-galactosidase gene under control of an SV-40 promotor. Two different monoclonal antibodies of a different subclass, IgM and IgG, were examined for their targeting ability using immunoliposomes. Cultured C6 glioma (specific target cell type) and NIH 3T3 (control cell type, fibroblast) cells were transfected using these immunoliposomes. Results indicate a three-fold increase in transfection by the glial specific immunoliposomes, "gliasomes", in glial cell culture over control liposomes. Gliasomes were exposed to NIH 3T3 cells and showed no enhanced transfection over control liposomes. Gliasomes were tested for their specificity by the addition of excess antibody to the cell culture in order to saturate specific receptors on C6 glioma cells. Results indicate a reduced transfection, nearly three-fold, in cells that were saturated with excess antibody prior to exposure to the immunoliposomes.
免疫脂质体是用一种针对神经胶质细胞的特异性抗体构建的。它们被用于检测细胞类型质粒转染的特异性和效率。脂质体含有一个受SV - 40启动子控制的β - 半乳糖苷酶基因。使用免疫脂质体检测了两种不同亚类的单克隆抗体IgM和IgG的靶向能力。使用这些免疫脂质体转染培养的C6胶质瘤（特异性靶细胞类型）和NIH 3T3（对照细胞类型，成纤维细胞）细胞。结果表明，在神经胶质细胞培养中，神经胶质特异性免疫脂质体“神经胶质体”的转染率比对照脂质体增加了三倍。将神经胶质体暴露于NIH 3T3细胞，与对照脂质体相比，未显示出增强的转染。通过向细胞培养物中添加过量抗体以饱和C6胶质瘤细胞上的特异性受体，来测试神经胶质体的特异性。结果表明，在暴露于免疫脂质体之前用过量抗体饱和的细胞中，转染率降低了近三倍。
