甲状腺功能亢进和甲状腺功能减退所引起的肉碱棕榈酰转移酶-I mRNA丰度的变化与活性变化平行。
Changes in carnitine palmitoyltransferase-I mRNA abundance produced by hyperthyroidism and hypothyroidism parallel changes in activity.
作者信息
Mynatt R L, Park E A, Thorngate F E, Das H K, Cook G A
机构信息
Department of Pharmacology, University of Tennessee, Memphis 38163.
出版信息
Biochem Biophys Res Commun. 1994 Jun 15;201(2):932-7. doi: 10.1006/bbrc.1994.1791.
To study the regulation of carnitine palmitoyltransferase-I by thyroid hormone, a cDNA was obtained by PCR amplification of DNA obtained by reverse transcription of rat liver RNA. CPT-I mRNA abundance was measured in livers of hyperthyroid, euthyroid and hypothyroid rats. In hypothyroid rats, the CPT-I mRNA levels decreased 40-fold relative to that of the hyperthyroid animals. These changes paralleled alterations in enzyme activity. These data suggest that CPT-I is regulated at the transcriptional level by thyroid hormone.
为研究甲状腺激素对肉碱棕榈酰转移酶-I（CPT-I）的调节作用，通过对大鼠肝脏RNA反转录得到的DNA进行PCR扩增获得了一条cDNA。测定了甲状腺功能亢进、甲状腺功能正常和甲状腺功能减退大鼠肝脏中CPT-I mRNA的丰度。在甲状腺功能减退的大鼠中，CPT-I mRNA水平相对于甲状腺功能亢进的动物降低了40倍。这些变化与酶活性的改变平行。这些数据表明CPT-I在转录水平受甲状腺激素调节。
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