Dallocchio F, Tomasi M, Bellini T
Dipartimento di Biochimica e Biologia Molecolare, Università di Ferrara, Italy.
Biochem Biophys Res Commun. 1994 Jun 15;201(2):988-93. doi: 10.1006/bbrc.1994.1799.
The Sendai virus envelope contains two glycoproteins: the fusion (F) protein and the hemagglutinin-neuraminidase (HN). Inactivation of F causes the loss of fusogenic activity and an increase of the neuraminidase activity of HN. After inactivation of F, HN can be inhibited by fetuin or asialofetuin, as already observed on the water-soluble, C-terminal fragment of HN (Dallocchio, F., Bellini, T., Martuscelli, G., Baiocchi, M., & Tomasi, M. (1991) Biochem. Int. 25, 663-668). Disruption of viral envelopes by detergents does not affect the neuraminidase activity of virions containing inactive F, while it causes an increase of the neuraminidase activity in native virions. Reconstitution of HN into liposomes is accompanied by a decrease of enzymatic activity, due to the random inside-outside distribution of the protein. However, the decrease of the neuraminidase activity is higher in liposomes containing both HN and F. These data suggest that F inhibits the neuraminidase activity of HN.
融合（F）蛋白和血凝素神经氨酸酶（HN）。F蛋白失活会导致融合活性丧失以及HN神经氨酸酶活性增加。F蛋白失活后，HN可被胎球蛋白或去唾液酸胎球蛋白抑制，这已在HN的水溶性C末端片段上观察到（达洛乔，F.，贝利尼，T.，马尔图塞利，G.，白奥基，M.，& 托马西，M.（1991年）《生物化学国际》25，663 - 668）。用去污剂破坏病毒包膜不会影响含有失活F的病毒粒子的神经氨酸酶活性，而会导致天然病毒粒子中神经氨酸酶活性增加。将HN重组到脂质体中会伴随着酶活性降低，这是由于蛋白质随机的内外分布所致。然而，在同时含有HN和F的脂质体中，神经氨酸酶活性的降低幅度更大。这些数据表明F抑制HN的神经氨酸酶活性。
