Seto E, Lewis B, Shenk T
Department of Cellular & Structural Biology, University of Texas Health Science Center, San Antonio 78245-3207.
Nature. 1993 Sep 30;365(6445):462-4. doi: 10.1038/365462a0.
A basal level of transcription is usually observed when all but a small region of DNA has been deleted from a eukaryotic gene promoter. These promoter elements, which are necessary and sufficient for specific transcription initiation, are referred to as minimal or core promoter elements. One element that is commonly present in a core promoter is the initiator. It has been demonstrated that the presence of Sp1 binding sites can greatly enhance the level of transcription initiation at initiator elements. A binding site for the YY1 transcription factor, located at the initiation site of the adeno-associated virus P5 promoter, functions as an initiator element; a synergistic enhancement of its activity is observed in vitro when upstream Sp1 binding sites are present. Here we report that this synergistic activation probably occurs through protein-protein interactions.
当从真核基因启动子中删除除一小部分DNA以外的所有区域时，通常会观察到基础转录水平。这些对特定转录起始来说必要且充分的启动子元件，被称为最小或核心启动子元件。核心启动子中通常存在的一个元件是起始子。已经证明，Sp1结合位点的存在可以极大地提高起始子元件处的转录起始水平。位于腺相关病毒P5启动子起始位点的YY1转录因子结合位点，作为一个起始子元件发挥作用；当存在上游Sp1结合位点时，在体外观察到其活性的协同增强。在此我们报告，这种协同激活可能通过蛋白质-蛋白质相互作用发生。
