Le Quan Sang K H, Kunes J, Zicha J, Vincent M, Sassard J, Devynck M A
URA CNRS 1482, Necker Medical School, Paris, France.
Am J Hypertens. 1994 Mar;7(3):276-81. doi: 10.1093/ajh/7.3.276.
The altered membrane microviscosity demonstrated in various cells of spontaneously hypertensive rats (SHR) and essential hypertensive (EH) patients has been proposed to play an important role in the pathogenesis of genetic forms of hypertension. The aim of this study was to evaluate possible changes of membrane microviscosity in platelets and red cell ghosts of Lyon hypertensive (LH) and normotensive (LN) rats. Both erythrocyte and platelet membranes of LH rats had a clear tendency to reduced DPH fluorescence anisotropy reflecting the decreased core membrane microviscosity. On the other hand, there were no changes in TMA-DPH fluorescence anisotropy that characterizes the dynamic properties of the outer membrane leaflet. DPH, but not TMA-DPH, anisotropy correlated negatively with blood pressure. This was true for both red cell ghosts and platelets. Membrane microviscosity had no significant relationship to plasma cholesterol or triglycerides. In platelets, TMA-DPH anisotropy correlated positively with cytosolic free calcium concentration ([Ca2+]i). A similar trend was observed in erythrocytes. In contrast, DPH anisotropy had an inverse relationship to platelet [Ca2+]i. It can be concluded that the alterations of membrane microviscosity seen in LH rats are completely different from those reported in SHR animals and that surface and core membrane microviscosity differ in their relationship to blood pressure and [Ca2+]i.
自发性高血压大鼠（SHR）和原发性高血压（EH）患者的多种细胞中所表现出的膜微粘度改变，被认为在遗传性高血压的发病机制中起重要作用。本研究的目的是评估里昂高血压（LH）大鼠和正常血压（LN）大鼠的血小板和红细胞影中膜微粘度的可能变化。LH大鼠的红细胞膜和血小板膜均有明显降低DPH荧光各向异性的趋势，这反映了核心膜微粘度的降低。另一方面，表征外膜小叶动态特性的TMA-DPH荧光各向异性没有变化。DPH而非TMA-DPH的各向异性与血压呈负相关。红细胞影和血小板均如此。膜微粘度与血浆胆固醇或甘油三酯无显著关系。在血小板中，TMA-DPH各向异性与胞质游离钙浓度（[Ca2+]i）呈正相关。在红细胞中也观察到类似趋势。相反，DPH各向异性与血小板[Ca2+]i呈负相关。可以得出结论，LH大鼠中所见的膜微粘度改变与SHR动物中报道的完全不同，并且表面膜和核心膜微粘度在与血压和[Ca2+]i的关系上有所不同。
