来自抗豆象野生普通豆的一种α-淀粉酶抑制剂的cDNA序列及推导的一级结构
cDNA sequence and deduced primary structure of an alpha-amylase inhibitor from a bruchid-resistant wild common bean.
作者信息
Suzuki K, Ishimoto M, Kitamura K
机构信息
Cooperated Graduate School, University of Tsukuba, Ibaraki, Japan.
出版信息
Biochim Biophys Acta. 1994 Jun 12;1206(2):289-91. doi: 10.1016/0167-4838(94)90221-6.
alpha-Amylase inhibitor-2 (alpha AI-2), a seed storage protein present in a bruchid-resistant wild common bean (Phaseolus vulgaris), inhibits the growth of bruchid pests. The authors isolated and determined the sequence of an 852 nucleotide cDNA, designated as alpha ai2, and found it to contain a 720 base open reading frame (ORF). This ORF encodes a 240 amino-acid alpha AI-2 polypeptide 75.8% identical with alpha-amylase inhibitor-1 (alpha AI-1) and 50.6-55.6% with arcelin-1, phytohemagglutinin (PHA)-L and PHA-E of common bean. The high degree of sequence homology suggests that there is an evolutionary relationship among these genes.
α-淀粉酶抑制剂-2（α AI-2）是一种存在于抗豆象野生普通菜豆（Phaseolus vulgaris）中的种子贮藏蛋白，可抑制豆象害虫的生长。作者分离并确定了一个852个核苷酸的cDNA序列，命名为α ai2，发现它包含一个720个碱基的开放阅读框（ORF）。该ORF编码一个240个氨基酸的α AI-2多肽，与α-淀粉酶抑制剂-1（α AI-1）的同源性为75.8%，与普通菜豆的arcelin-1、植物血凝素（PHA）-L和PHA-E的同源性为50.6 - 55.6%。高度的序列同源性表明这些基因之间存在进化关系。
Zotero 插件