凝集素对培养的胚胎成纤维细胞中糖胺聚糖代谢的年龄相关影响。

Age related effects of lectins on GAG metabolism in cultured embryonic fibroblasts.

作者信息

Becchetti E, Locci P, Marinucci L, Lilli C, Pezzetti F, Carinci P

机构信息

Dipartimento di Medicina Sperimentale, Università di Perugia, Italy.

出版信息

Cell Mol Biol (Noisy-le-grand). 1994 Mar;40(2):183-92.

PMID:8003949

Abstract

Lectins (WGA, SBA or PNA) were administered to 7 and 14 days chick embryo fibroblasts to evaluate their ability to influence GAG cellular and extracellular accumulation. HA and sulphated GAG were differently affected by lectins. WGA addition to culture medium enhanced HA and sulphated GAG in the two examined compartments. SBA and PNA increased sulphated GAG only in the cellular pool with a consequent reduction of secreted GAG. As far as HA is concerned, SBA and PNA promoted its accumulation in the extracellular compartment. NH4Cl administration indicated a decline of GAG degradation in the course of development in presence of lectins. Generally the effect of lectins was greater in the youngest stage: showing that lectins were able to bind 7 and 14 days-old fibroblasts differently.

摘要

将凝集素（小麦胚凝集素、大豆凝集素或花生凝集素）作用于7日龄和14日龄鸡胚成纤维细胞，以评估它们影响糖胺聚糖在细胞内和细胞外积累的能力。透明质酸（HA）和硫酸化糖胺聚糖受凝集素的影响不同。向培养基中添加小麦胚凝集素可增加两个检测隔室中的透明质酸和硫酸化糖胺聚糖。大豆凝集素和花生凝集素仅增加细胞池中的硫酸化糖胺聚糖，从而导致分泌的糖胺聚糖减少。就透明质酸而言，大豆凝集素和花生凝集素促进其在细胞外隔室中的积累。氯化铵的施用表明在存在凝集素的情况下，糖胺聚糖降解在发育过程中有所下降。一般来说，凝集素在最年轻阶段的作用更大：表明凝集素与7日龄和14日龄成纤维细胞的结合方式不同。