[The effect of Staphylococcus aureus vaccine on professional phagocytes in vitro and in vivo].

Effect of Staphylococcus aureus vaccine on metabolic and functional activities of polymorphonuclear leucocytes and guinea pig macrophages was observed. The tested vaccine following a short contact with human polymorphonuclear leucocytes isolated from peripheral blood inhibited candidacidal and phagocytic activities, whereas the phagocytic index stayed unchanged. The tested vaccine stimulated the INT-reductase activity and superoxide production, as well as extracellular liberation of lysosome enzymes from polymorphonuclear leucocytes. Application of the Staphylococcus aureus vaccine to guinea pigs 35, 30, 28, and 23 days prior to the acquirement of peritoneal macrophages, augmented, as compared to the control group, the amount of peritoneal macrophages and their phagocytic activity. Candidacidal and metabolic activities stayed unchanged. The results imply that the contact of phagocytic cells with the antigen, affects their biochemical and functional activities. Activities are dependent of the length of the period during which the antigen stimulation takes place, and of its quantity. Examination of phagocytes can be recommended for adjustment of both, vaccine doses and intervals between their individual applications. (Tab. 5, Ref. 19.).