Kishko Ia H, Vasylenko M I, Kovalenko E O, Pidhors'kyĭ V S
Institute of Microbiology and Virology, National Academy of Sciences, Kyiv, Ukraine.
Mikrobiol Z. 1998 Jan-Feb;60(1):36-42.
Intramuscular and intraperitoneal introduction of Bacillus subtilis sp. lectin to mongrel white mice is responsible for the increasing of values of functional activity of peripheral blood phagocytes and peritoneal macrophages of these animals. These results confirmed similar data obtained in vitro. Bactericidal activity of monocytes and neutrophils significantly increased at minimal doses of lectin (0.1-0.5 microgram/animal). But this increase was lower at middle doses (0.5-5.0 micrograms/animal) and again increased at higher ones (5.0-25.0 micrograms/animal). Dose-dependent effect of lectin on macrophages was similar, but it was observed at lower concentrations. Administration of lectin did not give increase of functional reserve of studied cells, but was responsible for the increase of the phagocyte number and phagocytic index of monocytes and neutrophils at relatively middle doses (1.0-5.0 micrograms/animal). Phagocytic activity of macrophages did not increase. Thus, lectin of B. subtilis sp. is the agent non-toxic during studies in vivo, stimulating functional activity of phagocytes and macrophages.
将枯草芽孢杆菌凝集素经肌肉注射和腹腔注射到杂种白色小鼠体内，会导致这些动物外周血吞噬细胞和腹腔巨噬细胞的功能活性值增加。这些结果证实了在体外获得的类似数据。在凝集素的最小剂量（0.1 - 0.5微克/动物）下，单核细胞和中性粒细胞的杀菌活性显著增加。但在中等剂量（0.5 - 5.0微克/动物）时这种增加较低，而在较高剂量（5.0 - 25.0微克/动物）时又再次增加。凝集素对巨噬细胞的剂量依赖性效应相似，但在较低浓度时就可观察到。凝集素的施用并未使所研究细胞的功能储备增加，但在相对中等剂量（1.0 - 5.0微克/动物）时会导致单核细胞和中性粒细胞的吞噬细胞数量及吞噬指数增加。巨噬细胞的吞噬活性并未增加。因此，枯草芽孢杆菌凝集素在体内研究期间是无毒的，可刺激吞噬细胞和巨噬细胞的功能活性。
