Mulira G L, Saunders J R
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon.
Can J Vet Res. 1994 Apr;58(2):104-8.
We measured antibody levels in serum and cervicovaginal mucus (CVM) of four heifers vaccinated with two inoculations of killed Ureaplasma diversum strain 2312 in incomplete Freund's adjuvant (IFA) two weeks apart, and six heifers given a placebo. Two weeks later, the vaccinates and four placebo heifers, were challenged by intravaginal inoculation with 6.4 x 10(8) colony-forming units of the homologous U. diversum strain. The remaining two placebo heifers served as unvaccinated, unchallenged controls. Antibody levels in serum and CVM of all heifers were determined by an enzyme-linked immunosorbent assay (ELISA). Vaccination stimulated specific IgG1 and IgG2 responses in serum and CVM but only a slight IgM and no IgA response. In both vaccinate and placebo heifers, subsequent intravaginal challenge resulted in a granular vulvitis (GV) with a predominant IgA response in the CVM. The GV gradually subsided during the 35 day observation period but ureaplasmas were consistently demonstrated by culture. We concluded that subcutaneous vaccination stimulated a specific, albeit nonprotective, IgG response in serum and CVM. In contrast, vaginal infection primarily induced a mucosal IgA response.
我们检测了四头小母牛血清和宫颈阴道黏液（CVM）中的抗体水平。这四头小母牛每隔两周用不完全弗氏佐剂（IFA）接种两次灭活的解脲脲原体2312菌株，还有六头小母牛接受了安慰剂处理。两周后，接种疫苗的小母牛和四头接受安慰剂的小母牛通过阴道接种6.4×10⁸个同源解脲脲原体菌株的菌落形成单位进行攻毒。其余两头接受安慰剂的小母牛作为未接种疫苗、未攻毒的对照。所有小母牛血清和CVM中的抗体水平通过酶联免疫吸附测定（ELISA）来确定。接种疫苗刺激了血清和CVM中特异性IgG1和IgG2反应，但仅有轻微的IgM反应且无IgA反应。在接种疫苗和接受安慰剂的小母牛中，随后的阴道攻毒均导致颗粒性外阴炎（GV），CVM中以IgA反应为主。在35天的观察期内，GV逐渐消退，但通过培养始终能检测到脲原体。我们得出结论，皮下接种疫苗刺激了血清和CVM中特异性的、尽管无保护作用的IgG反应。相比之下，阴道感染主要诱导黏膜IgA反应。
