Callender T, el-Naggar A K, Lee M S, Frankenthaler R, Luna M A, Batsakis J G
Department of Otolaryngology, Baylor College of Medicine, Houston, Texas.
Cancer. 1994 Jul 1;74(1):152-8. doi: 10.1002/1097-0142(19940701)74:1<152::aid-cncr2820740124>3.0.co;2-k.
Abnormalities in chromosome 11q13 regions have been frequently found in head and neck squamous carcinoma. Recent studies indicate that the PRAD-1 (also CCND1), which encodes cyclin D1, is a putative oncogene that is an important component of this region.
DNA was extracted from 32 snap-frozen specimens from primary head and neck squamous carcinomas. DNA from peripheral blood lymphocytes, normal mucosa, and salivary gland tissue were used as controls. A genomic DNA probe containing the first exon of PRAD-1 was used for hybridization with specimen DNAs by the Southern technique. A 5.6-kb genomic DNA probe of immunoglobulin heavy chain was used as an internal standard for assessing PRAD-1 amplification.
Eleven (34.4%) squamous carcinoma specimens showed PRAD-1 amplification (2- to 10-fold). Although no significant statistical correlation among amplification status, grade stage, and DNA ploidy was observed in this small cohort, amplification was more noted in high grade, high stage, and aneuploid tumors. A highly statistical correlation between PRAD-1 amplification and proliferative activity was noted (P > 0.001).
The results of this study indicate that PRAD-1 amplification appears to be a late event in the tumorigenesis of head and neck carcinoma and is associated often with a subset of aggressive tumors and high proliferation neoplasms.
11q13区域的染色体异常在头颈部鳞状细胞癌中经常被发现。最近的研究表明，编码细胞周期蛋白D1的PRAD-1（也称为CCND1）是该区域的一个假定癌基因，是该区域的重要组成部分。
从32例原发性头颈部鳞状细胞癌的速冻标本中提取DNA。外周血淋巴细胞、正常黏膜和唾液腺组织的DNA用作对照。使用含有PRAD-1第一外显子的基因组DNA探针，通过Southern技术与标本DNA进行杂交。免疫球蛋白重链的5.6kb基因组DNA探针用作评估PRAD-1扩增的内标。
11例（34.4%）鳞状细胞癌标本显示PRAD-1扩增（2至10倍）。虽然在这个小队列中未观察到扩增状态、分级分期和DNA倍体之间有显著的统计学相关性，但在高级别、高分期和非整倍体肿瘤中扩增更为明显。PRAD-1扩增与增殖活性之间存在高度统计学相关性（P>0.001）。
本研究结果表明，PRAD-1扩增似乎是头颈部癌发生过程中的一个晚期事件，且常与侵袭性肿瘤和高增殖性肿瘤的一个亚组相关。
